{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T23:40:37Z","timestamp":1771630837052,"version":"3.50.1"},"reference-count":33,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,1]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Childhood tuberculosis meningitis is a severe form of tuberculosis with high morbidity and mortality. The diagnosis is frequently missed and delayed due to lack of sensitive tests like acid-fast bacilli (AFB) smear and delayed results by culture.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aims.<\/jats:bold> To compare the role of IS6110 and protein antigen b PCR in cerebrospinal fluid (CSF) for rapid diagnosis of tuberculous meningitis (TBM) in children.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Forty-five cases of TBM and 20 controls were enrolled in this prospective study.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> The mean ages of cases and controls were 4.2\u00b10.5\u2009years and 4.5\u00b10.7\u2009years, respectively. In the TBM group, two-thirds of the children were &lt;4\u2009years of age, and 62\u200a% were males. Sensitivities of AFB smear examination, L\u00f6wenstein\u2013Jensen (LJ) medium and bactenecin (BACTEC) culture in cases were 4.4, 0 and 2.2%, respectively. The protein antigen b PCR was most sensitive as it was positive in 35 (77.8\u200a%) of TBM patients; IS6110 PCR was positive in 27 (60\u200a%) patients. Both PCR-based tests had higher positivity than conventional tests and BACTEC culture. No significant difference was seen between the PCR tests. Excellent agreement was observed between both PCR-based tests as they were concordant for 26 positive samples and 35 negative samples.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Protein b PCR is a sensitive and rapid method for the diagnosis of TBM (sensitivity 77.8\u200a%). Both PCRs were more sensitive than smear, LJ and BACTEC. The specificity of both PCR was 100\u200a%.<\/jats:p>","DOI":"10.1099\/jmm.0.001220","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T15:15:26Z","timestamp":1593011726000},"page":"979-985","source":"Crossref","is-referenced-by-count":4,"title":["Comparative evaluation of IS6110 and protein antigen b PCR in cerebrospinal fluid for rapid diagnosis of tuberculous meningitis in children"],"prefix":"10.1099","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1388-2933","authenticated-orcid":false,"given":"Sikha","family":"Agarwal","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Postgraduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arushi Gahlot","family":"Saini","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Postgraduate Institute of Medical Education and Research, Chandigarh, 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