{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:09:39Z","timestamp":1762272579297,"version":"3.40.5"},"reference-count":13,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,8,1]]},"abstract":"\n Introduction<\/jats:bold>. Empirical vancomycin (VAN) treatment failure for methicillin-resistant \n \n Staphylococcus aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (MRSA) bacteraemia, with significantly higher mortality, has been reported for MRSA strains with reduced VAN susceptibility.<\/jats:p>\n \n Aim<\/jats:bold>. Our goal was to study the effect of sub-culture on VAN minimum inhibitory concentration (MIC) values compared to direct susceptibility of MRSA-positive blood cultures.<\/jats:p>\n \n Methodology<\/jats:bold>. Using 19 MRSA-positive blood cultures and 19 seeded MRSA-positive blood cultures, we compared the VAN MICs from direct susceptibility testing of MRSA-positive blood cultures and MRSA sub-cultured from positive blood cultures.<\/jats:p>\n \n Results<\/jats:bold>. In comparing direct VAN MICs from MRSA-positive blood cultures and standard agar dilution, nearly half of the MICs from agar dilution were lower, with one sample decreasing from 1.5 to 0.75\u2009\u00b5g\u2009ml\u22121<\/jats:sup>. Furthermore, in seeded blood cultures, 80\u200a% or more showed lower values from standard agar dilution compared to direct VAN MICs.<\/jats:p>\n \n Conclusion<\/jats:bold>. Our results reveal a trend towards lower MICs after positive blood culture isolates are sub-cultured. Some clinical failures among MRSA infections treated with VAN may result from this phenomenon.<\/jats:p>","DOI":"10.1099\/jmm.0.001225","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T13:15:08Z","timestamp":1594214108000},"page":"1095-1099","source":"Crossref","is-referenced-by-count":1,"title":["Does sub-culturing of positive MRSA blood cultures affect vancomycin MICs?"],"prefix":"10.1099","volume":"69","author":[{"given":"Izumo","family":"Kanesaka","sequence":"first","affiliation":[{"name":"Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1, Miyama, Funabashi-shi, Chiba 274-8510, Japan"},{"name":"Department of Infection Control and Prevention, Faculty of Nursing, Toho University, 4-16-20, Omori-nishi, Ota-ku, Tokyo 143-0015, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takamitsu","family":"Ito","sequence":"additional","affiliation":[{"name":"Higashiosaka City Medical Center, 3-4-5, Nishiiwata, Higashiosaka-shi, Osaka 578-8588, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritsuko","family":"Shishido","sequence":"additional","affiliation":[{"name":"LSI Medience Corporation, 3-30-1, Shimura, Itabashi-ku, Tokyo 174-8555, Japan"},{"name":"Toho University Sakura Medical Center, 564-1, Shimoshizu, Sakura-shi, Chiba 285-8741, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7369-2173","authenticated-orcid":false,"given":"Makoto","family":"Nagashima","sequence":"additional","affiliation":[{"name":"Toho University Sakura Medical Center, 564-1, Shimoshizu, Sakura-shi, Chiba 285-8741, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiko","family":"Kanayama Katsuse","sequence":"additional","affiliation":[{"name":"Department of Infection Control and Prevention, Faculty of Nursing, Toho University, 4-16-20, Omori-nishi, Ota-ku, Tokyo 143-0015, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Infection Control and Prevention, Faculty of Nursing, Toho University, 4-16-20, Omori-nishi, Ota-ku, Tokyo 143-0015, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7703-3079","authenticated-orcid":false,"given":"Shingo","family":"Fujisaki","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1, Miyama, Funabashi-shi, Chiba 274-8510, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Intetsu","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Infection Control and Prevention, Faculty of Nursing, Toho University, 4-16-20, Omori-nishi, Ota-ku, Tokyo 143-0015, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","article-title":"https:\/\/janis.mhlw.go.jp\/report\/open_report\/2018\/3\/2\/zen_Open_Report_201800.pdf. 2018"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/40.1.135"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa025025"},{"volume-title":"Performance Standards for Antimicrobial Susceptibility Testing; 26th Informational Supplement. CLSI document M100-S26","year":"2016","key":"R4"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1086\/524667"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00448-13"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.2.272-277.2000"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01720.x"},{"volume-title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically\u221211th ed. CLSI document M07Ed11","year":"2018","key":"R9"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00904-08"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01571-14"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.5-14.2003"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-010-0086-0"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001225?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T14:19:42Z","timestamp":1599142782000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,1]]},"references-count":13,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2020,8,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.001225","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"type":"print","value":"0022-2615"},{"type":"electronic","value":"1473-5644"}],"subject":[],"published":{"date-parts":[[2020,8,1]]}}}