{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:42:06Z","timestamp":1780548126886,"version":"3.54.1"},"reference-count":52,"publisher":"Microbiology Society","issue":"9","funder":[{"name":"Higher Education Comprehensive Award fund","award":["2018-702090250401"],"award-info":[{"award-number":["2018-702090250401"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> PCV2 is a DNA virus that exists widely in pigs and has caused great economic losses to the pig industry worldwide. In the existing commercial PCV2 enzyme-linked immunosorbent assay (ELISA) kits both natural infection with PCV2 and vaccine immunization produce results that are positive for PCV2 Cap antibodies and therefore they cannot diagnose PCV2 infection in immunized pig farms.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> To establish a PCV2 non-structural protein antibody detection method that distinguishes between antibodies resulting from natural prior exposure (infection) and those induced by subunit vaccine immunization.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Based on the non-structural Rep\u2032 protein, we established an indirect ELISA (iELISA) using sera from guinea pigs and piglets.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> The results for iELISA for guinea pig serum showed that animals vaccinated with a whole-virus inactivated PCV2 vaccine had 100\u200a% (10\/10) Cap antibody positivity and 100\u200a% (10\/10) Rep\u2032 antibody positivity. Guinea pigs vaccinated with a recombinant subunit PCV2 vaccine had 100\u200a% (10\/10) Cap antibody positivity, while no (0\/10) guinea pigs were Rep\u2032 antibody-positive. The combined detection results for the Rep\u2032 iELISA and a PCV2 Antibody Test kit (Commercial) showed that pigs vaccinated with a whole-virus inactivated PCV2 vaccine or PCV2 SD\/2017 had 100\u200a% (5\/5) Cap antibody positivity and 100\u200a% (5\/5) Rep\u2032 antibody positivity. Pigs vaccinated with a recombinant subunit PCV2 vaccine had 100\u200a% (5\/5) Cap antibody positivity, while no (0\/10) pigs were Rep\u2032 antibody-positive.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> This paper describes an effective iELISA method that can distinguish natural infection with PCV2 (Cap and Rep positive) or inoculation with a whole-virus inactivated vaccine (Cap and Rep positive) from subunit vaccine immunization (Cap-positive, Rep-negative). These comparative assays could be very useful in the control of PCV2 in pig herds.<\/jats:p>","DOI":"10.1099\/jmm.0.001230","type":"journal-article","created":{"date-parts":[[2020,8,19]],"date-time":"2020-08-19T16:16:23Z","timestamp":1597853783000},"page":"1183-1196","source":"Crossref","is-referenced-by-count":6,"title":["Establishment of a Rep\u2032 protein antibody detection method to distinguish natural infection with PCV2 from subunit vaccine immunization"],"prefix":"10.1099","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5051-5649","authenticated-orcid":false,"given":"Qingqing","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Biomedicine, Yangtze University, JingZhou, HuBei 434025, PR China"},{"name":"College of Life Sciences, Yangtze University, JingZhou, HuBei 434025, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Rong","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Yangtze University, JingZhou, HuBei 434025, PR China"},{"name":"Yebio Bioengineering Co. 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