{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:08:40Z","timestamp":1747181320977,"version":"3.40.5"},"reference-count":34,"publisher":"Microbiology Society","issue":"1","funder":[{"name":"The National Institute of Health, Lima-Peru, has supported this study."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,1]]},"abstract":"<jats:p>Asymptomatic carriers are a likely source of transmission of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1926\" xlink:type=\"simple\">Neisseria meningitidis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> to close contacts who are placed at a higher risk for invasive meningococcal disease (IMD). Although <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1926\" xlink:type=\"simple\">N. meningitidis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> ciprofloxacin-resistance is rare, there have been an increase in the reports of resistant isolates mainly in patients diagnosed with IMD, and little is known about the <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1926\" xlink:type=\"simple\">N. meningitidis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> ciprofloxacin-resistance in the carrier populations. We performed a pharyngeal carriage study during a 2017 military setting outbreak in Peru, caused by a ciprofloxacin-resistant <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1926\" xlink:type=\"simple\">N. meningitidis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> B. The isolates analysed came from two hospitalized cases and six asymptomatic carriers. Whole-genome sequence-based analysis was performed and showed that strains carrying the Thr<jats:bold>91<\/jats:bold>Ile mutation, in the gene encoding for subunit A of DNA gyrase (<jats:italic>gyr<\/jats:italic>A), were responsible for the fluoroquinolone resistance (MICs \u22650.256 \u00b5g ml<jats:sup>\u22121<\/jats:sup>) and were closely related to highly virulent strains from France, Norway and the UK. Phylogenetic analysis of the <jats:italic>gyr<\/jats:italic>A gene revealed that likely these Peruvian isolates acquired resistance through horizontal gene transfer from <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1924\" xlink:type=\"simple\">Neisseria lactamica<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. Our study provides evidence for the emergence and propagation of ciprofloxacin-resistant <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1926\" xlink:type=\"simple\">N. meningitidis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> B from asymptomatic carriers, and recommends the introduction of serogroup B vaccines for high-risk populations.<\/jats:p>","DOI":"10.1099\/jmm.0.001245","type":"journal-article","created":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T16:23:23Z","timestamp":1605543803000},"source":"Crossref","is-referenced-by-count":2,"title":["Emergence of ciprofloxacin-resistant Neisseria meningitidis B from asymptomatic carriers during an outbreak in Peru, 2017"],"prefix":"10.1099","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6843-3206","authenticated-orcid":false,"given":"Eduardo","family":"Juscamayta-L\u00f3pez","sequence":"first","affiliation":[{"name":"Centro Nacional de Salud P\u00fablica, Instituto Nacional de Salud, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8347-6853","authenticated-orcid":false,"given":"Faviola","family":"Valdivia","sequence":"additional","affiliation":[{"name":"Centro Nacional de Salud P\u00fablica, Instituto Nacional de Salud, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Morales","sequence":"additional","affiliation":[{"name":"Centro Nacional de Salud P\u00fablica, Instituto Nacional de Salud, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Fernando","family":"Donaires","sequence":"additional","affiliation":[{"name":"Centro Nacional de Salud P\u00fablica, Instituto Nacional de Salud, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5554-3542","authenticated-orcid":false,"given":"Victor","family":"Fiestas-Sol\u00f3rzano","sequence":"additional","affiliation":[{"name":"Centro Nacional de Salud P\u00fablica, Instituto Nacional de Salud, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2048-4626","authenticated-orcid":false,"given":"Marianela","family":"Or\u00e9","sequence":"additional","affiliation":[{"name":"Epidemiolog\u00eda, Comando de Salud del Ej\u00e9rcito, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Pachas","sequence":"additional","affiliation":[{"name":"Centro Nacional de Salud P\u00fablica, Instituto Nacional de Salud, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Le\u00f3n-Janampa","sequence":"additional","affiliation":[{"name":"Centro Nacional de Salud P\u00fablica, Instituto Nacional de Salud, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1437-5607","authenticated-orcid":false,"given":"Ronnie","family":"Gavil\u00e1n","sequence":"additional","affiliation":[{"name":"Centro Nacional de Salud P\u00fablica, Instituto Nacional de Salud, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","article-title":"Meningococcal serogroups AMD survellance: a systematic review and survey","volume":"9","author":"Peterson","year":"2019"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(15)00217-0"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1038\/s41429-018-0125-0"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.32.2.323-330.1994"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ram.2015.08.004"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800050196"},{"key":"R7","article-title":"Antibiotics for preventing meningococcal infections (Review)","author":"ZT","year":"2013","journal-title":"The Cochrane Collaboration"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.03.027"},{"year":"2011","key":"R9","article-title":"Laboratory methods for the diagnosis of meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae laboratory methods for the diagnosis of meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae"},{"journal-title":"Eighteenth Informational Supplement","article-title":"Performance standards for antimicrobial susceptibility testing","year":"2019","key":"R10"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1017"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009490"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx610"},{"key":"R14","doi-asserted-by":"crossref","DOI":"10.3201\/eid1410.080252","article-title":"Serogroup A Neisseria meningitidis with reduced susceptibility to ciprofloxacin","volume":"14","author":"Strahilevitz","year":"2008","journal-title":"Emerg Infect Dis"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0806414"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.1.192-195.2000"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/49.3.545"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61016-2"},{"key":"R19","first-page":"8","article-title":"Meningococcal carriage prevalence in university students, 18\u201324 years of age in Santiago, Chile","author":"Rodr\u00edguez","year":"2014","journal-title":"Vaccine"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135497"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45529-0"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.3201\/eid1206.051297"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000685"},{"key":"R24","first-page":"1614","article-title":"Ciprofloxacin- resistant Neisseria meningitidis, Delhi, India","volume":"13","author":"Singhal","year":"2007","journal-title":"Emerging"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06094-11"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2015.01.004"},{"key":"R27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.26633\/RPSP.2019.10","article-title":"Grupo del laboratorio VIMENE Paraguay. Genotipos de Neisseria meningitidis aislados de pacientes con enfermedad meningoc\u00f3cica en Paraguay, 1996-2015","volume":"43","author":"Le\u00f3n","year":"2019","journal-title":"Rev Panam Salud Publica"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.18.5551-5556.1999"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01331-10"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5459.1809"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019751108"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.078600-0"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026061"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0806414"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001245?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,29]],"date-time":"2021-01-29T13:12:29Z","timestamp":1611925949000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,1]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.001245","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"type":"print","value":"0022-2615"},{"type":"electronic","value":"1473-5644"}],"subject":[],"published":{"date-parts":[[2021,1,1]]}}}