{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T21:11:02Z","timestamp":1790457062654,"version":"4.1.0"},"reference-count":47,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"\n Introduction.<\/jats:bold> \n \n \n Staphylococcus aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> is a skin and mucous commensal bacterium of warm-blooded animals. In humans, the nose is the main ecological niche of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, and nasal carriage is a risk factor for developing an endogenous infection. \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> nasal colonization is a multifactorial process, involving inter-species interactions among the nasal microbiota.<\/jats:p>\n \n Aims.<\/jats:bold> The objectives of this study were to characterize the microbiota of carriers and non-carriers of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and to demonstrate the importance of inter-species relationships in the adhesion of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, a key step in nasal colonization.<\/jats:p>\n \n Methodology.<\/jats:bold> First, we characterized the nasal microbiota from 30 \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> carriers and non-carriers by a culturomic approach. We then evaluated the adhesion of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, first alone and then along with other bacteria of the nasal microbiota. To do that, we used an in vitro<\/jats:italic> model to measure the interactions among bacteria in the presence of epithelial cells.<\/jats:p>\n \n Results.<\/jats:bold> Analysis of the nasal microbiota of the carriers and non-carriers of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> made it possible to observe that each microbiota has specific features in terms of composition. However, this composition differs significantly between carriers and non-carriers mainly through two bacterial groups: coagulase-negative staphylococci and corynebacteria. In a second part, adhesion of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> to epithelial cells showed competition between \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and these bacteria, suggesting a limitation of nasal colonization by \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>.<\/jats:p>\n \n Conclusion.<\/jats:bold> These findings demonstrate the existence of a negative correlation between \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and other species which inhibits adhesion and could limit nasal colonization.<\/jats:p>","DOI":"10.1099\/jmm.0.001248","type":"journal-article","created":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T17:33:41Z","timestamp":1599759221000},"page":"1253-1261","source":"Crossref","is-referenced-by-count":10,"title":["Adhesion of Staphylococcus aureus to epithelial cells: an in vitro approach to study interactions within the 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