{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:49:53Z","timestamp":1790693393585,"version":"4.1.0"},"reference-count":18,"publisher":"Microbiology Society","issue":"1","funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["18K07120"],"award-info":[{"award-number":["18K07120"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["18K07121"],"award-info":[{"award-number":["18K07121"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["19K16652"],"award-info":[{"award-number":["19K16652"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["19KK0203"],"award-info":[{"award-number":["19KK0203"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["20K16252"],"award-info":[{"award-number":["20K16252"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["20fk0108061h0303"],"award-info":[{"award-number":["20fk0108061h0303"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"name":"JU Research Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,1]]},"abstract":"\n \n \n Pseudomonas asiatica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n Pseudomonas monteilii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, belonging to the \n \n Pseudomonas putida<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> phylogenetic group, are occasionally isolated from clinical samples, partly because they are often misidentified as \n \n P. putida<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> in clinical laboratories. There are five reports describing carbapenem-resistant clinical isolates of these species. Carbapenem-resistant strains of \n \n P. asiatica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n P. monteilii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> were isolated from stool samples. These isolates were sequenced using Illumina MiSeq and reidentified using average nucleotide identity (ANI) based on comparisons of their whole-genome sequences using the OrthoANI algorithm. The clonal relatedness of the isolates was assessed by pulse-field gel electrophoresis (PFGE). The size of plasmids conveying bla<\/jats:italic>\n VIM-2<\/jats:sub> was examined by Southern blotting. A total of six carbapenem-resistant clinical isolates of \n \n P. asiatica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (two isolates) and \n \n P. monteilii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (four isolates) were obtained from stool samples from five patients in a Japanese hospital. All isolates harboured blaVIM-2<\/jats:sub>\n <\/jats:italic>. The two isolates of \n \n P. asiatica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> had a different pattern in the PFGE analysis, with both having a 23\u2009kb plasmid. Of the four isolates of \n \n P. monteilii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> with similar patterns in the PFGE analysis, three had 320\u2009kb plasmids and one had a 240\u2009kb plasmid. The genetic environments of the 320\/240\u2009kb and 23\u2009kb plasmids differed. The results strongly indicated that carbapenem-resistant \n \n P. asiatica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n P. monteilii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> producing metallo-\u03b2-lactamase are emerging in Japan. This is the first report of carbapenem-resistant \n \n P. asiatica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n P. monteilii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> in Japan.<\/jats:p>","DOI":"10.1099\/jmm.0.001258","type":"journal-article","created":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T15:34:06Z","timestamp":1606145646000},"source":"Crossref","is-referenced-by-count":14,"title":["Emergence of clinical isolates of Pseudomonas asiatica and Pseudomonas monteilii from Japan harbouring an acquired gene encoding a carbapenemase VIM-2"],"prefix":"10.1099","volume":"70","author":[{"given":"Mari","family":"Tohya","sequence":"first","affiliation":[{"name":"Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohei","family":"Uechi","sequence":"additional","affiliation":[{"name":"Division of Clinical Laboratory and Blood Transfusion, University Hospital of the Ryukyus, Okinawa, Japan"},{"name":"Department of Infectious Diseases, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Tada","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomomi","family":"Hishinuma","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0262-0401","authenticated-orcid":false,"given":"Takeshi","family":"Kinjo","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeya","family":"Ohshiro","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Naha City Hospital, Okinawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6688-9875","authenticated-orcid":false,"given":"Shiro","family":"Maeda","sequence":"additional","affiliation":[{"name":"Division of Clinical Laboratory and Blood Transfusion, University Hospital of the Ryukyus, Okinawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruo","family":"Kirikae","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3423-8343","authenticated-orcid":false,"given":"Jiro","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03264-15"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.003316"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2019.04.004"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004199"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00475-19"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-3-846"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1080\/17843286.2020.1740464"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.redar.2019.06.003"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04639-14"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-017-2600-9"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00716-11"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr294"},{"key":"R13","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing; 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