{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T04:25:20Z","timestamp":1779337520634,"version":"3.51.4"},"reference-count":27,"publisher":"Microbiology Society","issue":"2","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"crossref","award":["1009855"],"award-info":[{"award-number":["1009855"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,2,1]]},"abstract":"Introduction.<\/jats:bold>Moraxella bovoculi<\/jats:ext-link><\/jats:named-content><\/jats:italic>is frequently isolated from the eyes of cattle with infectious bovine keratoconjunctivitis (IBK; pinkeye). As withM. bovis,<\/jats:italic>which has been causally linked to IBK,M. bovoculi<\/jats:ext-link><\/jats:named-content><\/jats:italic>expresses an RTX (repeats in the structural toxin) cytotoxin that is related toM. bovis<\/jats:ext-link><\/jats:named-content><\/jats:italic>cytotoxin. Pilin, another pathogenic factor inM. bovis<\/jats:ext-link><\/jats:named-content><\/jats:italic>, is required for corneal attachment. Seven antigenically distinct pilin serogroups have been described inM. bovis<\/jats:ext-link><\/jats:named-content><\/jats:italic>.<\/jats:p>Hypothesis\/Gap Statement.<\/jats:bold>Multiple different serogroups exist amongst type IV pilin encoded byM. bovis<\/jats:ext-link><\/jats:named-content><\/jats:italic>, however, it is not known whetherM. bovoculi<\/jats:ext-link><\/jats:named-content><\/jats:italic>exhibits a similar degree of diversity in type IV pilin that it encodes.<\/jats:p>Aim.<\/jats:bold>This study was done to characterize a structural pilin (PilA) encoded byM. bovoculi<\/jats:ext-link><\/jats:named-content><\/jats:italic>isolated from cases of IBK to determine if diversity exists amongst PilA sequences.<\/jats:p>Methodology.<\/jats:bold>Ninety-four isolates ofM. bovoculi<\/jats:ext-link><\/jats:named-content><\/jats:italic>collected between 2002 and 2017 from 23 counties throughout California and from five counties in four other Western states were evaluated.<\/jats:p>Results.<\/jats:bold>DNA sequencing and determination of deduced amino acid sequences revealed ten (designated groups A through J) unique PilA sequences that were ~96.1\u201399.3\u200a%\u2009identical. Pilin groups A and C matched previously reported putative PilA sequences fromM. bovoculi<\/jats:ext-link><\/jats:named-content><\/jats:italic>isolated from IBK-affected cattle in the USA (Virginia, Nebraska, and Kansas) and Asia (Kazakhstan). The ten pilin sequences identified were only ~74\u201376\u200a%\u2009identical to deduced amino acid sequences of putative pilin proteins identified from the previously reported whole-genome sequences ofM. bovoculi<\/jats:ext-link><\/jats:named-content><\/jats:italic>derived from deep nasopharyngeal swabs of IBK-asymptomatic cattle.<\/jats:p>Conclusions.<\/jats:bold>Compared to the diversity reported between structural pilin proteins amongst different serogroups ofM. bovis<\/jats:ext-link><\/jats:named-content><\/jats:italic>,M. bovoculi<\/jats:ext-link><\/jats:named-content><\/jats:italic>PilA from geographically diverse isolates derived from IBK-affected cattle are more conserved.<\/jats:p>","DOI":"10.1099\/jmm.0.001293","type":"journal-article","created":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T15:17:48Z","timestamp":1609946268000},"source":"Crossref","is-referenced-by-count":17,"title":["Relatedness of type IV pilin PilA amongst geographically diverse Moraxella bovoculi isolated from cattle with infectious bovine keratoconjunctivitis"],"prefix":"10.1099","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4903-8267","authenticated-orcid":false,"given":"John A.","family":"Angelos","sequence":"first","affiliation":[{"name":"Department of Medicine & Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristin A.","family":"Clothier","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology & Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8137-5642","authenticated-orcid":false,"given":"Regina L.","family":"Agulto","sequence":"additional","affiliation":[{"name":"Department of Medicine & Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3311-5037","authenticated-orcid":false,"given":"Boguslav","family":"Mandzyuk","sequence":"additional","affiliation":[{"name":"Department of Medicine & Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5394-5196","authenticated-orcid":false,"given":"Morten","family":"Tryland","sequence":"additional","affiliation":[{"name":"Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Framstredet 39, N-9037 Troms\u00f8, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64333-0"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638714551403"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2014.11.009"},{"key":"R4","first-page":"761","article-title":"Infectious bovine keratoconjunctivitis. 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