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The repositioning of drugs, which is characterized by the use of drugs already on the market for other purposes, together with molecular-docking methods can be used aiming at the faster development of new antifungals to combat micro-organisms. This study aimed to evaluate the antifungal effect of diazepam on mature<jats:italic>C. albicans<\/jats:italic>biofilms<jats:italic>in vitro<\/jats:italic>and its action on biofilm in formation, as well as its mechanism of action and interaction with structures related to the adhesion of<jats:italic>C. albicans<\/jats:italic>, ALS3 and SAP5. To determine the MIC, the broth microdilution test was used according to protocol M27-A3 (CLSI, 2008).<jats:italic>In vitro<\/jats:italic>biofilm formation tests were performed using 96-well plates, followed by molecular-docking protocols to analyse the binding agent interaction with ALS3 and SAP5 targets. The results indicate that diazepam has antimicrobial activity against planktonic cells of<jats:italic>Candida<\/jats:italic>spp. and<jats:italic>C. albicans<\/jats:italic>biofilms, interacting with important virulence factors related to biofilm formation (ALS3 and SAP5). In addition, treatment with diazepam triggered a series of events in<jats:italic>C. albicans<\/jats:italic>cells, such as loss of membrane integrity, mitochondrial depolarization and increased production of EROs, causing DNA damage and consequent cell apoptosis.<\/jats:p>","DOI":"10.1099\/jmm.0.001308","type":"journal-article","created":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T17:20:01Z","timestamp":1612891201000},"source":"Crossref","is-referenced-by-count":9,"title":["Diazepam\u2019s antifungal activity in fluconazole-resistant Candida spp. and biofilm inhibition in C. albicans: evaluation of the relationship with the proteins ALS3 and SAP5"],"prefix":"10.1099","volume":"70","author":[{"given":"Lisandra","family":"Juv\u00eancio da Silva","sequence":"first","affiliation":[{"name":"Drug Research and Development Center, Federal University of Cear\u00e1, Fortaleza, CE, Brazil"},{"name":"School of Pharmacy, Laboratory for Bioprospection of Antimicrobial Molecules (LABIMAN), 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