{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T11:25:57Z","timestamp":1771673157425,"version":"3.50.1"},"reference-count":45,"publisher":"Microbiology Society","issue":"3","funder":[{"DOI":"10.13039\/501100004263","name":"FAPERGS","doi-asserted-by":"crossref","award":["16\/2551-0000517-6"],"award-info":[{"award-number":["16\/2551-0000517-6"]}],"id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,1]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Onychomycosis infections currently show a significant increase, affecting about 10\u200a% of the world population. <jats:italic>Trichophyton rubrum<\/jats:italic> is the main agent responsible for about 80\u200a% of the reported infections. The clinical cure for onychomycosis is extremely difficult and effective new antifungal therapy is needed.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis\/Gap Statement.<\/jats:bold> \n                  <jats:italic>Ex vivo<\/jats:italic> onychomycosis models using porcine hooves can be an excellent alternative for evaluating the efficacy of new anti-dermatophytic agents in a nail lacquer.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> Evaluation of the effectiveness of a nail lacquer containing a quinoline derivative on an <jats:italic>ex vivo<\/jats:italic> onychomycosis model using porcine hooves, as well as the proposal of a plausible antifungal mechanism of this derivative against dermatophytic strains.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> The action mechanism of a quinoline derivative was evaluated through the sorbitol protection assay, exogenous ergosterol binding, and the determination of the dose-response curves by time-kill assay. Scanning electron microscopy evaluated the effect of the derivative in the fungal cells. The efficacy of a quinoline-derivative nail lacquer on an <jats:italic>ex vivo<\/jats:italic> onychomycosis model using porcine hooves was evaluated as well.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> The quinoline derivative showed a time-dependent fungicidal effect, demonstrating reduction and damage in the morphology of dermatophytic hyphae. In addition, the <jats:italic>ex vivo<\/jats:italic> onychomycosis model was effective in the establishment of infection by <jats:italic>T. rubrum<\/jats:italic>.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Treatment with the quinoline-derivative lacquer showed a significant inhibitory effect on <jats:italic>T. rubrum<\/jats:italic> strain in this infection model. Finally, the compound presents high potential for application in a formulation such as nail lacquer as a possible treatment for dermatophytic onychomycosis.<\/jats:p>","DOI":"10.1099\/jmm.0.001314","type":"journal-article","created":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T10:21:43Z","timestamp":1611742903000},"source":"Crossref","is-referenced-by-count":2,"title":["Ex vivo potential of a quinoline-derivative nail lacquer as a new alternative for dermatophytic onychomycosis treatment"],"prefix":"10.1099","volume":"70","author":[{"given":"Gabriella da Rosa Monte","family":"Machado","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Microbiologia Agr\u00edcola e do Ambiente, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4259-6327","authenticated-orcid":false,"given":"Bruna","family":"Pippi","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Microbiologia Agr\u00edcola e do Ambiente, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9904-792X","authenticated-orcid":false,"given":"Simone","family":"Berlitz","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Nanotecnologia Farmac\u00eautica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise","family":"Diedrich","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Defferrari","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6040-3704","authenticated-orcid":false,"given":"William","family":"Lopes","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone Cristina Baggio","family":"Gnoatto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Nanotecnologia Farmac\u00eautica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8607-7612","authenticated-orcid":false,"given":"Irene Clemes","family":"Kulkamp-Guerreiro","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Nanotecnologia Farmac\u00eautica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilene Henning","family":"Vainstein","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8885-8586","authenticated-orcid":false,"given":"Mickael","family":"Jean","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Rennes 1, Institut des Sciences Chimiques de Rennes - UMR CNRS 6226 Equipe COrInt, F- 35000 Rennes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Van de Weghe","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Rennes 1, Institut des Sciences Chimiques de Rennes - UMR CNRS 6226 Equipe COrInt, F- 35000 Rennes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saulo Fernandes","family":"de Andrade","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Microbiologia Agr\u00edcola e do Ambiente, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre Meneghello","family":"Fuentefria","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Microbiologia Agr\u00edcola e do Ambiente, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1007\/s12281-015-0231-4"},{"key":"R2","first-page":"1","article-title":"The host range of dermatophytes, it is at all possible? Phenotypic evaluation of the keratinolytic activity of Trichophyton verrucosum clinical isolates","volume":"2018","author":"Gnat","year":"2018","journal-title":"Mycoses"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cpm.2016.02.001"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12633"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2015.03.009"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12532"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.20546\/ijcmas.2017.611.152"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-016-0071-y"},{"key":"R9","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/978-3-319-72156-9_18","volume-title":"Gender and Dermatology","author":"Shemer","year":"2018"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/j.simyco.2018.02.004"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjid.2013.08.005"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1177\/1203475417724439"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2013.08.032"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2015.04.043"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2016.03.008"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2014.07.044"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2014.930454"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2014.08.008"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-015-1323-y"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2016.12.023"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.10.046"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2018.05.031"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1007\/s42770-020-00348-4"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2013.01.015"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1111\/ics.12142"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08549.x"},{"key":"R27","first-page":"M38","volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous fungi; Approved Standard","year":"2008"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2016.1158286"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1021\/np070660i"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myx003"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1017\/S1431927615005474"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12961"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.15.34"},{"key":"R34","first-page":"115","article-title":"Introducing new antimalarial analogues of chloroquine and amodiaquine: a narrative review","volume":"42","author":"Parhizgar","year":"2017","journal-title":"Iran J Med Sci"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1708153114"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw426"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2016.10.017"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2018.04.010"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3557-z"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2014.11.017"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1007\/s13555-016-0093-x"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.11.019"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12475"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-018-0242-0"},{"key":"R45","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/978-3-319-39424-4_10","volume-title":"Antibiotic and Antifungal Therapies in Dermatology","author":"Sigurgeirsson","year":"2016"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001314?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T09:24:14Z","timestamp":1621502654000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,1]]},"references-count":45,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,3,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.001314","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,1]]}}}