{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T02:07:49Z","timestamp":1762999669267,"version":"3.37.3"},"reference-count":19,"publisher":"Microbiology Society","issue":"3","funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP20fk0108061"],"award-info":[{"award-number":["JP20fk0108061"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP20fk0108093"],"award-info":[{"award-number":["JP20fk0108093"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP20fk0108139"],"award-info":[{"award-number":["JP20fk0108139"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP20fk0108133"],"award-info":[{"award-number":["JP20fk0108133"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP20wm0225008"],"award-info":[{"award-number":["JP20wm0225008"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,1]]},"abstract":"<jats:p>Tigecycline is a last-resort antimicrobial used to treat multidrug-resistant Gram-negative bacterial infections. One of the common antimicrobial resistance mechanisms is the efflux pump system composed of membrane protein complexes to excrete xenobiotic substrates. Recently, a novel gene cluster, <jats:italic>tmexCD1-toprJ1<\/jats:italic>, encoding the resistance\u2013nodulation\u2013cell division (RND) efflux pump was identified on plasmids in <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10789\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> isolates in China. TMexCD1-TOprJ1 was found to be capable of excreting multiple antimicrobials, including tigecycline, which contributed to the strain's resistance. In this study, we identified <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10789\" xlink:type=\"simple\">K. pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> isolates harbouring the <jats:italic>tmexCD1-toprJ1<\/jats:italic> genes outside of China for the first time. Two tigecycline-resistant <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10789\" xlink:type=\"simple\">K. pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> isolates belonging to ST273 by multilocus sequence typing were collected from different patients in a medical institution in Hanoi, Vietnam, in 2015. Whole-genome sequence analysis revealed that these isolates harboured a 288.0\u2009kb <jats:italic>tmexCD1-toprJ1<\/jats:italic>\u2013carrying plasmid with IncFIB and IncHI1B replicons. The <jats:italic>tmexCD1-toprJ1<\/jats:italic> gene cluster was surrounded by several mobile gene elements, including IS<jats:italic>26<\/jats:italic>, and the plasmids had high sequence identity with that of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10789\" xlink:type=\"simple\">K. pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> isolated in China. Our finding suggests that the horizontal spread of tigecycline resistance mediated by <jats:italic>tmexCD1-toprJ1<\/jats:italic>\u2013carrying plasmids has occurred in Vietnam and other countries, and raises concern about the further global dissemination.<\/jats:p>","DOI":"10.1099\/jmm.0.001320","type":"journal-article","created":{"date-parts":[[2021,3,11]],"date-time":"2021-03-11T10:15:57Z","timestamp":1615457757000},"source":"Crossref","is-referenced-by-count":20,"title":["Emergence of a plasmid-borne tigecycline resistance in Klebsiella pneumoniae in Vietnam"],"prefix":"10.1099","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3115-0645","authenticated-orcid":false,"given":"Aki","family":"Hirabayashi","sequence":"first","affiliation":[{"name":"AMR Research Center, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Van Thi Thu","family":"Ha","sequence":"additional","affiliation":[{"name":"Microbiology Department, Hospital 103, Military Medical University, Hanoi, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"An Van","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Present address: Department of Viral Infection and International Health, Graduate School of Medical Sciences, Kanazawa University, Ishikawa, Japan"},{"name":"Microbiology Department, Hospital 103, Military Medical University, Hanoi, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5878-9150","authenticated-orcid":false,"given":"Son Thai","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Microbiology Department, Hospital 103, Military Medical University, Hanoi, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1233-6842","authenticated-orcid":false,"given":"Keigo","family":"Shibayama","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8975-2193","authenticated-orcid":false,"given":"Masato","family":"Suzuki","sequence":"additional","affiliation":[{"name":"AMR Research Center, National Institute of Infectious Diseases, Tokyo, 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