{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T13:20:14Z","timestamp":1760016014962,"version":"3.40.5"},"reference-count":26,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,1]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> \n                  <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.6313\" xlink:type=\"simple\">Mycobacterium abscessus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> complex (MABC) is an infectious agent associated with macrolide resistance and treatment failure.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis\/Gap Statement.<\/jats:bold> Despite drug-susceptibility testing for MABC isolates including clarithromycin (CAM), long-term treatment with azithromycin (AZM) for MABC disease is recommended.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> We compared phenotypic and genotypic resistance to AZM and CAM in clinical isolates and evaluated the accumulation of intrinsic macrolide resistance (AIM) and morphological changes by macrolides exposure.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Forty-nine isolates were characterized regarding <jats:italic>erm<\/jats:italic>(41) sequevars. Sequencing data were compared to the nucleotide sequence of <jats:italic>rrl<\/jats:italic> and <jats:italic>whiB7<\/jats:italic>. The AIM MIC was performed in three reference strains and 15 isolates were randomized [each set of five isolates with <jats:italic>\n                     <jats:named-content content-type=\"subspecies\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.22536\" xlink:type=\"simple\">M. abscessus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> subsp. <jats:italic>\n                     <jats:named-content content-type=\"subspecies\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.22536\" xlink:type=\"simple\">abscessus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (MAA) T28, MAA C28 and subsp. <jats:italic>massiliense<\/jats:italic> (MAM)].<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> The 49 isolates were distributed as 24 MAA T28, 5 MAA C28 and 20 MAM. The MIC<jats:sub>50<\/jats:sub> values to CAM at day 3 in MAA T28, C28 and MAM were 1, 0.12 and 0.12\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup>, while those at day 14 were 32, 0.5 and 0.12\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup>, respectively. The AZM-MIC<jats:sub>50<\/jats:sub> values at day 3 of the above isolates were 4, 0.25 and 0.5\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup>, while those at day 14 were &gt;64, 0.5 and 0.5\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup>, respectively. Neither mutations in <jats:italic>rrl<\/jats:italic> of MAA T28 with acquired resistance nor deletions in <jats:italic>whiB7<\/jats:italic> of MAA T28 without inducible resistance were observed . For AIM MIC, MAA T28 showed that the time-to-detection of AZM resistance was significantly faster over that of CAM (<jats:italic>P<\/jats:italic>&lt;0.05). Morphological changes were not determined in all isolates.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Our findings did not support the suggestion for the preferential use of AZM for, at least, MAA T28 disease due to the high-level MIC value and the increased AIM. The long duration of AZM-based treatment eventually may favour the emergence of isolates with a high-level of intrinsic resistance.<\/jats:p>","DOI":"10.1099\/jmm.0.001326","type":"journal-article","created":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T09:25:40Z","timestamp":1613035540000},"source":"Crossref","is-referenced-by-count":7,"title":["Comparison of drug-susceptibility patterns and gene sequences associated with clarithromycin and azithromycin resistance in Mycobacterium abscessus complex isolates and evaluation of the accumulation of intrinsic macrolide resistance"],"prefix":"10.1099","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8197-3829","authenticated-orcid":false,"given":"Shiomi","family":"Yoshida","sequence":"first","affiliation":[{"name":"Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, Sakai-shi, Osaka, 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