{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:36:16Z","timestamp":1772156176757,"version":"3.50.1"},"reference-count":15,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T00:00:00Z","timestamp":1628121600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008982","name":"Qatar National Research Fund","doi-asserted-by":"crossref","award":["RRC-2-032"],"award-info":[{"award-number":["RRC-2-032"]}],"id":[{"id":"10.13039\/100008982","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004252","name":"Qatar University","doi-asserted-by":"crossref","award":["QUERG-CMED-2020-2"],"award-info":[{"award-number":["QUERG-CMED-2020-2"]}],"id":[{"id":"10.13039\/501100004252","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004252","name":"Qatar University","doi-asserted-by":"crossref","award":["M-QJRC-2020-5 grant."],"award-info":[{"award-number":["M-QJRC-2020-5 grant."]}],"id":[{"id":"10.13039\/501100004252","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,5]]},"abstract":"<jats:p>\n                    Several studies have investigated the effect of repeated freeze\u2013thaw (F\/T) cycles on RNA detection for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, no data are available regarding the effect of repeated F\/T cycles on SARS-CoV-2 antibody detection in serum. We investigated the effect of multiple F\/T cycles on anti-SARS-CoV-2 IgG detection using an ELISA test targeting the nucleocapsid antibodies. Ten positive and 1 negative SARS-CoV-2 IgG sera from 11 participants, in replicates of 5, were subjected to a total of 16\u2009F\/T cycles and stored at 4\u2009\u00b0C until tested by ELISA. Statistical analysis was performed to test for F\/T cycle effect. None of the 10 positive sera became negative after 16\u2009F\/T cycles. There was no significant difference in the OD average reading between the first and last F\/T cycles, except for one serum with a minimal decline in the OD. The random effect linear regression of log (OD) on the number of cycles showed no significant trend, with a slope consistent with zero (B=\u22120.0001; 95\u200a%\u2009CI \u22120.0008; 0.0006;\n                    <jats:italic>P<\/jats:italic>\n                    -value=0.781). These results suggest that multiple F\/T cycles had no effect on the ability of the ELISA assay to detect SARS-CoV-2 IgG antibodies.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.001402","type":"journal-article","created":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T10:16:59Z","timestamp":1628245019000},"source":"Crossref","is-referenced-by-count":9,"title":["Effect of multiple freeze\u2013thaw cycles on the detection of anti-SARS-CoV-2 IgG antibodies"],"prefix":"10.1099","volume":"70","author":[{"given":"Farah M.","family":"Shurrab","sequence":"first","affiliation":[{"name":"Biomedical Research Center, Qatar University, Doha 2713, Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0487-3365","authenticated-orcid":false,"given":"Duaa W.","family":"Al-Sadeq","sequence":"additional","affiliation":[{"name":"College of Medicine, Member of QU Health, Qatar University, Doha, Qatar"},{"name":"Biomedical Research Center, Qatar University, Doha 2713, Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fathima","family":"Amanullah","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, Qatar University, Doha 2713, Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0717-0574","authenticated-orcid":false,"given":"Salma N.","family":"Younes","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, Qatar University, Doha 2713, Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadeel","family":"Al-Jighefee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, College of Health Sciences, Member of QU Health, Qatar University, Doha, Qatar"},{"name":"Biomedical Research Center, Qatar University, Doha 2713, Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5241-944X","authenticated-orcid":false,"given":"Nadin","family":"Younes","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, Qatar University, Doha 2713, Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soha R.","family":"Dargham","sequence":"additional","affiliation":[{"name":"World Health Organization Collaborating Centre for Disease Epidemiology Analytics on HIV\/AIDS, Sexually Transmitted Infections, and Viral Hepatitis, Weill Cornell Medicine\u2013Qatar, Cornell University, Qatar Foundation \u2013 Education City, Doha, Qatar"},{"name":"Infectious Disease Epidemiology Group, Weill Cornell Medicine\u2013Qatar, Cornell University, Qatar Foundation \u2013 Education City, Doha, Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadi M.","family":"Yassine","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, College of Health Sciences, Member of QU Health, Qatar University, Doha, Qatar"},{"name":"Biomedical Research Center, Qatar University, Doha 2713, Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0790-0506","authenticated-orcid":false,"given":"Laith J.","family":"Abu Raddad","sequence":"additional","affiliation":[{"name":"Infectious Disease Epidemiology Group, Weill Cornell Medicine\u2013Qatar, Cornell University, Qatar Foundation \u2013 Education City, Doha, Qatar"},{"name":"Department of Healthcare Policy and Research, Weill Cornell Medicine, Cornell University, New York, USA"},{"name":"World Health Organization Collaborating Centre for Disease Epidemiology Analytics on HIV\/AIDS, Sexually Transmitted Infections, and Viral Hepatitis, Weill Cornell Medicine\u2013Qatar, Cornell University, Qatar Foundation \u2013 Education City, Doha, Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9252-1038","authenticated-orcid":false,"given":"Gheyath K.","family":"Nasrallah","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, Qatar University, Doha 2713, Qatar"},{"name":"Department of Biomedical Science, College of Health Sciences, Member of QU Health, Qatar University, Doha, Qatar"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.5935\/1676-2444.20170038"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000036"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2013.1263"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.11613\/bm.2013.009"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1002\/jps.23473"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1089\/cpt.2008.0012"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1002\/rth2.12071"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.10.1.19-21.2003"},{"key":"R9","first-page":"193","article-title":"Biologic banking in cohort studies, with special reference to blood","volume":"67","author":"Petrakis","year":"1985","journal-title":"Natl Cancer Inst 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