{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:46:24Z","timestamp":1790390784682,"version":"4.1.0"},"reference-count":48,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T00:00:00Z","timestamp":1628121600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,5]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold>\n                  <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.38692\" xlink:type=\"simple\">Staphylococcus coagulans<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (formerly <jats:italic>\n                     <jats:named-content content-type=\"subspecies\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5271\" xlink:type=\"simple\">Staphylococcus schleiferi<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> subsp. <jats:italic>\n                     <jats:named-content content-type=\"subspecies\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5271\" xlink:type=\"simple\">coagulans<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>) is a common commensal and opportunistic pathogen of companion dogs. It carries a range of antimicrobial resistance genes and is an occasional zoonotic pathogen.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis\/Gap Statement.<\/jats:bold> Despite the potential insight offered by genome sequencing into the biology of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.38692\" xlink:type=\"simple\">S. coagulans<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, few genomes are currently available for study.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> To sequence and analyse <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.38692\" xlink:type=\"simple\">S. coagulans<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> genomes to improve understanding of this organism\u2019s molecular epidemiology, antimicrobial resistance and bacterium\u2013host interactions.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Twenty-five genomes of clinical isolates collected at a veterinary referral hospital in Scotland, UK, were sequenced with Illumina technology. These genomes were analysed by a series of bioinformatics tools along with 16 previously sequenced genomes.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Phylogenetic comparison of the 41 genomes shows that the current <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.38692\" xlink:type=\"simple\">S. coagulans<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> phylogeny is dominated by clades of closely related isolates, at least one of which has spread internationally. Ten of the 11 methicillin-resistant <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.38692\" xlink:type=\"simple\">S. coagulans<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> genomes in this collection of 41 encoded the <jats:italic>mecA<\/jats:italic> promoter and gene mutations that are predicted to render the isolates susceptible to penicillins in the presence of clavulanic acid, a feature only described to date in methicillin-resistant <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.11043\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. Seven such isolates were from the current study and, in line with the genome-based prediction, all were susceptible to amoxicillin\/clavulanic acid <jats:italic>in vitro. S. coagulans<\/jats:italic> shared very few highly conserved virulence-associated genes with <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.9497\" xlink:type=\"simple\">Staphylococcus pseudintermedius<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, another common commensal and opportunistic canine pathogen.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> The availability of a further 25 genome sequences from clinical <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.38692\" xlink:type=\"simple\">S. coagulans<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> isolates will aid in better understanding the epidemiology, bacterial\u2013host interactions and antimicrobial resistance of this opportunistic pathogen.<\/jats:p>","DOI":"10.1099\/jmm.0.001407","type":"journal-article","created":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T15:17:04Z","timestamp":1629904624000},"source":"Crossref","is-referenced-by-count":7,"title":["Genomic epidemiology of the opportunistic pathogen Staphylococcus coagulans from companion dogs"],"prefix":"10.1099","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1880-0095","authenticated-orcid":false,"given":"Gavin K.","family":"Paterson","sequence":"first","affiliation":[{"name":"Royal Dick School of Veterinary Studies and The Roslin Institute, University of Edinburgh, Edinburgh EH25 9RG, 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