{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:48:42Z","timestamp":1787010522250,"version":"build-2736575974"},"reference-count":50,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T00:00:00Z","timestamp":1634774400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Wairarapa Research Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,21]]},"abstract":"\n Introduction.<\/jats:bold>\n Antibiotic use, particularly amoxicillin-clavulanic acid in dairy farming, has been associated with an increased incidence of AmpC-hyperproducing\n \n \n Escherichia coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n .\n <\/jats:p>\n \n Gap statement.<\/jats:bold>\n There is limited information on the incidence of AmpC-hyperproducing\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n from seasonal pasture-fed dairy farms.\n <\/jats:p>\n \n Aim.<\/jats:bold>\n We undertook a New Zealand wide cross-sectional study to determine the prevalence of AmpC-producing\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n carried by dairy cattle.\n <\/jats:p>\n \n Methodology.<\/jats:bold>\n Paddock faeces were sampled from twenty-six dairy farms and were processed for the selective growth of both extended-spectrum beta-lactamase (ESBL)- and AmpC-producing\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n . Whole genome sequence analysis was carried out on 35 AmpC-producing\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n .\n <\/jats:p>\n \n Results.<\/jats:bold>\n No ESBL- or plasmid mediated AmpC-producing\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n were detected, but seven farms were positive for chromosomal mediated AmpC-hyperproducing\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n . These seven farms were associated with a higher usage of injectable amoxicillin antibiotics. Whole genome sequence analysis of the AmpC-producing\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n demonstrated that the same strain (<3\u2009SNPs difference) of\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ST5729 was shared between cows on a single farm. Similarly, the same strain (\u226415\u2009SNPs difference) of\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ST8977 was shared across two farms (separated by approximately 425\u2009km).\n <\/jats:p>\n \n Conclusion.<\/jats:bold>\n These results infer that both cow-to-cow and farm-to-farm transmission of AmpC-producing\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n has occurred.\n <\/jats:p>","DOI":"10.1099\/jmm.0.001447","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T12:15:28Z","timestamp":1634818528000},"source":"Crossref","is-referenced-by-count":9,"title":["The epidemiology of AmpC-producing Escherichia coli isolated from dairy cattle faeces on pasture-fed farms"],"prefix":"10.1099","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1449-7321","authenticated-orcid":false,"given":"Sara A.","family":"Burgess","sequence":"first","affiliation":[{"name":"Food System Integrity, Hopkirk Research Institute, AgResearch Ltd, Palmerston North, New Zealand"},{"name":"School of Veterinary Science, Massey University, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8197-3386","authenticated-orcid":false,"given":"Adrian L.","family":"Cookson","sequence":"additional","affiliation":[{"name":"Food System Integrity, Hopkirk Research Institute, AgResearch Ltd, Palmerston North, New Zealand"},{"name":"School of Veterinary Science, Massey University, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Brousse","sequence":"additional","affiliation":[{"name":"Present address: Lisa Brousse, BioMerieux, Grenoble, France"},{"name":"School of Veterinary Science, Massey University, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Ortolani","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture, Livestock and Food Supply, Brazil"},{"name":"School of Veterinary Science, Massey University, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7814-4341","authenticated-orcid":false,"given":"Jackie","family":"Benschop","sequence":"additional","affiliation":[{"name":"School of Veterinary Science, Massey University, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rukhshana","family":"Akhter","sequence":"additional","affiliation":[{"name":"School of Veterinary Science, Massey University, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gale","family":"Brightwell","sequence":"additional","affiliation":[{"name":"New Zealand Food Safety Science and Research Centre, Massey University, Palmerston North, New Zealand"},{"name":"Food System Integrity, Hopkirk Research Institute, AgResearch Ltd, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9865-0532","authenticated-orcid":false,"given":"Scott","family":"McDougall","sequence":"additional","affiliation":[{"name":"Cognosco Limited, Anexa Veterinary Services, Morrinsville, New Zealand"},{"name":"School of Veterinary Science, Massey University, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2017.11.004"},{"key":"R2","first-page":"205","article-title":"Consumer attitudes in Germany towards different dairy housing systems and their implications for the marketing of pasture raised milk","volume":"17","author":"Weinrich","year":"2014","journal-title":"Int Food Agribusiness Manag Rev"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv485"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2009.07.014"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01260"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.10.015"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-016-2752-3"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2014.0284"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-019-01180-3"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01580-09"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm538"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2017-13658"},{"key":"R13","article-title":"Antimicrobial bacteria in dairy cattle: A review. 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