{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T18:48:26Z","timestamp":1783968506594,"version":"3.55.0"},"reference-count":27,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,20]]},"abstract":"\n Introduction<\/jats:bold>\n \n .<\/jats:bold> Capnocytophaga<\/jats:italic> species are common inhabitants of the oral cavity and can be responsible for systemic diseases in immunocompromised patients with granulocytopenia. Furthermore, it has been reported that some clinical isolates of \n \n Capnocytophaga<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species produce extended-spectrum \u03b2-lactamases (ESBLs).<\/jats:p>\n \n Gap statement.<\/jats:bold> Information is lacking about the types of \u03b2-lactamase genes possessed by \n \n Capnocytophaga<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> spp. and the antimicrobial susceptibility of \n \n Capnocytophaga<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> spp. possessing each \u03b2-lactamase gene.<\/jats:p>\n \n Aim.<\/jats:bold> The aim of this study was to investigate the presence of \u03b2-lactamase genes in clinical strains of \u03b2-lactamase-producing \n \n Capnocytophaga<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species isolated from clinical samples acquired at Shinshu University Hospital and examine the antimicrobial susceptibility of those strains.<\/jats:p>\n \n Methodology.<\/jats:bold> The \u03b2-lactamase-producing \n \n Capnocytophaga<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species (n<\/jats:italic>=49) were obtained from clinical specimens. PCR assays were used to detect bla<\/jats:italic>\n CfxA<\/jats:sub>, bla<\/jats:italic>\n CSP<\/jats:sub>, bla<\/jats:italic>\n TEM<\/jats:sub>, bla<\/jats:italic>\n CepA\/CblA<\/jats:sub> and transposon Tn4555<\/jats:italic> genes. Southern hybridization assays were used to detect bla<\/jats:italic>\n CfxA<\/jats:sub> and bla<\/jats:italic>\n CSP<\/jats:sub>. The minimum inhibitory concentration of some \u03b2-lactams was determined using the E-test method.<\/jats:p>\n \n Results.<\/jats:bold> PCR analysis indicated that the bla<\/jats:italic>\n CfxA<\/jats:sub> gene was present in 15 (30.6\u200a%) and the bla<\/jats:italic>\n CSP<\/jats:sub> gene in 35 (69.3\u200a%) of the 49 \n \n Capnocytophaga<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strains investigated, . Both bla<\/jats:italic>\n CfxA<\/jats:sub> and bla<\/jats:italic>\n CSP<\/jats:sub> genes were detected in a \n \n Capnocytophaga gingivalis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strain. The PCR results were confirmed by Southern hybridization assays. Transposon Tn4555<\/jats:italic> was only detected in \n \n Capnocytophaga<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> spp. harbouring the bla<\/jats:italic>\n CfxA<\/jats:sub> gene. All the \u03b2-lactamase-producing \n \n Capnocytophaga<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates were susceptible to ceftazidime\u2013clavulanic acid, cefoxitin and imipenem. In contrast, most of the isolates were resistant to amoxicillin.<\/jats:p>\n \n Conclusions.<\/jats:bold> The clinical isolates of \n \n Capnocytophaga<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> spp. showed a high prevalence of the bla<\/jats:italic>\n CSP<\/jats:sub> gene in Japan. The presence of the bla<\/jats:italic>\n CSP<\/jats:sub> gene was distributed in \n \n Capnocytophaga sputigena<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> as well as other \n \n Capnocytophaga<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> spp. These results seem to suggest the dissemination of bla<\/jats:italic>\n CfxA<\/jats:sub> and bla<\/jats:italic>\n CSP<\/jats:sub> \u03b2-lactamase genes among \n \n Capnocytophaga<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species.<\/jats:p>","DOI":"10.1099\/jmm.0.001448","type":"journal-article","created":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T08:58:17Z","timestamp":1635238697000},"source":"Crossref","is-referenced-by-count":4,"title":["Antibiotic resistance and the presence of bla\n CfxA and bla\n CSP genes in \u03b2-lactamase-producing clinical Capnocytophaga isolates from a university hospital in Japan"],"prefix":"10.1099","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1504-5474","authenticated-orcid":false,"given":"Takehisa","family":"Matsumoto","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan"},{"name":"Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, 3-39-22 Showa-machi, Maebashi, Gunma 371-8514, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Matsumoto-Matsubara","sequence":"additional","affiliation":[{"name":"Clinical Investigation and Research Unit, Gunma University Hospital, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan"},{"name":"Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9011-5204","authenticated-orcid":false,"given":"Kazuki","family":"Horiuchi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6559-0011","authenticated-orcid":false,"given":"Eriko","family":"Arai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7266-2562","authenticated-orcid":false,"given":"Tatsuya","family":"Negishi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, 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