{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:07:14Z","timestamp":1788998834790,"version":"build-2803163510"},"reference-count":42,"publisher":"Microbiology Society","issue":"4","funder":[{"DOI":"10.13039\/501100004160","name":"Mazandaran University of Medical Sciences","doi-asserted-by":"crossref","award":["1597"],"award-info":[{"award-number":["1597"]}],"id":[{"id":"10.13039\/501100004160","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,22]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> \n                  <jats:italic>Aspergillus<\/jats:italic> sections <jats:italic>Flavi<\/jats:italic> and <jats:italic>Nigri<\/jats:italic> comprise clinically relevant and cryptic species that differ significantly in drug susceptibility, meaning that effective treatment depends on correct species identification.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis\/Gap Statement.<\/jats:bold> There are no comprehensive data for molecular identification and antifungal susceptibility testing (AFST) of clinically relevant and cryptic species of <jats:italic>Aspergillus<\/jats:italic> sections <jats:italic>Flavi<\/jats:italic> and <jats:italic>Nigri<\/jats:italic> as the main agents of invasive and non-invasive aspergillosis in Iran.<\/jats:p>\n               <jats:p> We aimed to perform molecular identification and AFST of 213 clinical <jats:italic>Aspergillus<\/jats:italic> isolates belonging to sections <jats:italic>Flavi<\/jats:italic> and <jats:italic>Nigri<\/jats:italic>.<\/jats:p>\n               <jats:p> Molecular identification of isolates was performed using sequencing of the \u03b2-tubulin gene and <jats:italic>in vitro<\/jats:italic> AFST was conducted according to the Clinical and Laboratory Standards Institute (CLSI) M38-A3 guidelines.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> The most common isolates in sections <jats:italic>Flavi<\/jats:italic> and <jats:italic>Nigri<\/jats:italic> were <jats:italic>Aspergillus flavus<\/jats:italic> (110\/113, 97.3\u200a%) and <jats:italic>Aspergillus tubingensis<\/jats:italic> (49\/100, 49.0\u200a%), respectively. A total of 62\/213 (29.1\u200a%) isolates belonging to cryptic species were identified; among them, <jats:italic>A. tubingensis<\/jats:italic> was the most prevalent (49\/62, 79.0%). <jats:italic>Aspergillus flavus<\/jats:italic> and <jats:italic>A. niger<\/jats:italic> isolates that responded to the minimum inhibitory concentrations (MICs) of itraconazole above the epidemiological cutoff values were the most frequently detected: 8\/110 (7.3\u200a%) and 3\/41 (7.3\u200a%), respectively. In section <jats:italic>Flavi<\/jats:italic>, <jats:italic>Aspergillus alliaceus<\/jats:italic> responded to amphotericin B at a high MIC (&gt;16 \u00b5g mL<jats:sup>\u22121<\/jats:sup>) and in section <jats:italic>Nigri<\/jats:italic>, one of the three <jats:italic>Aspergillus luchuensis\/awamori<\/jats:italic> isolates responded to itraconazole at an MIC &gt;16 \u00b5g ml<jats:sup>\u22121<\/jats:sup>. Interestingly, for all <jats:italic>Aspergillus welwitschiae<\/jats:italic> isolates, the MIC<jats:sub>50<\/jats:sub> and MIC<jats:sub>90<\/jats:sub> of itraconazole were both 16 \u00b5g ml<jats:sup>\u22121<\/jats:sup>.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> A considerable presence of <jats:italic>A. flavus<\/jats:italic> and <jats:italic>A. niger<\/jats:italic> isolates showing non-wild-type responses to azoles in clinical cases of aspergillosis indicates the importance of classifying clinical <jats:italic>Aspergillus<\/jats:italic> isolates at the species level and performing antifungal susceptibility testing on the isolates, which would ensure appropriate treatment.<\/jats:p>","DOI":"10.1099\/jmm.0.001480","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T15:18:04Z","timestamp":1650640684000},"source":"Crossref","is-referenced-by-count":21,"title":["Molecular identification and antifungal susceptibility of clinically relevant and cryptic species of Aspergillus sections Flavi and Nigri"],"prefix":"10.1099","volume":"71","author":[{"given":"Sanaz","family":"Nargesi","sequence":"first","affiliation":[{"name":"Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran"},{"name":"Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jalal","family":"Jafarzadeh","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology, School of Medicine, Babol University of Medical Sciences, Babol, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0590-1073","authenticated-orcid":false,"given":"Mohammad Javad","family":"Najafzadeh","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology, Faculty of 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