{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T15:50:27Z","timestamp":1782834627327,"version":"3.54.5"},"reference-count":25,"publisher":"Microbiology Society","issue":"5","license":[{"start":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T00:00:00Z","timestamp":1653955200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,31]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> The cycle threshold (Ct) value in real-time PCR (RT-PCR) is where a target-specific amplification signal becomes detectable and can infer viral load, risk of transmission and recovery. Use of Ct values in routine practice is uncommon.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap Statement.<\/jats:bold> There is a lack of routine use of Ct values when reporting RT-PCR results in routine practice.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> To automatically insert Ct values and interpretive comments when reporting SARS-CoV-2 RT-PCR to improve patient management.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Routine Ct values across three different RT-PCR platforms were reviewed for concordance at presentation and clearance in patients with COVID-19. An indicative threshold (IT) linked to viral clearance kinetics was defined at Ct30 to categorize Ct values as low and high, reflecting high and low viral loads respectively.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> The different gene targets of each platform showed high correlation and kappa score agreement (<jats:italic>P<\/jats:italic>&lt;0.001). Average Ct values were automatically generated with values \u2264Ct30 reported as positive and &gt;Ct30 as reactive; interpretive comments were added to all reports. The new reporting algorithm impacted on: physician interpretation of SARS-CoV-2 results; patient management and transfer; staff surveillance; length of stay in quarantine; and redefinition of patient recovery.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Incorporation of Ct values into routine practice is possible across different RT-PCR platforms and adds useful information for patient management. The use of an IT with interpretive comments improves clinical interpretation and could be a model for reporting other respiratory infections. Withholding Ct values wastes useful clinical data and should be reviewed by the profession, accreditation bodies and regulators.<\/jats:p>","DOI":"10.1099\/jmm.0.001499","type":"journal-article","created":{"date-parts":[[2022,5,16]],"date-time":"2022-05-16T16:17:24Z","timestamp":1652717844000},"source":"Crossref","is-referenced-by-count":12,"title":["Reporting of RT-PCR cycle threshold (Ct) values during the first wave of COVID-19 in Qatar improved result interpretation in clinical and public health settings"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0819-6103","authenticated-orcid":false,"given":"Peter V.","family":"Coyle","sequence":"first","affiliation":[{"name":"Biomedical Research Center, Member of QU Health, Qatar University, Doha, Qatar"},{"name":"Hamad Medical Corporation, Doha, Qatar"},{"name":"Wellcome-Wolfson Institute for Experimental Medicine, Queen's University, Belfast, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naema Hassan","family":"Al Molawi","sequence":"additional","affiliation":[{"name":"Hamad Medical Corporation, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed Ali Ben Hadj","family":"Kacem","sequence":"additional","affiliation":[{"name":"Hamad Medical Corporation, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reham Awni","family":"El Kahlout","sequence":"additional","affiliation":[{"name":"Hamad Medical Corporation, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Einas","family":"Al Kuwari","sequence":"additional","affiliation":[{"name":"Hamad Medical Corporation, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullatif","family":"Al Khal","sequence":"additional","affiliation":[{"name":"Hamad Medical Corporation, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5793-9591","authenticated-orcid":false,"given":"Imtiaz","family":"Gillani","sequence":"additional","affiliation":[{"name":"Hamad Medical Corporation, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0375-2713","authenticated-orcid":false,"given":"Andrew","family":"Jeremijenko","sequence":"additional","affiliation":[{"name":"Hamad Medical Corporation, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4493-3219","authenticated-orcid":false,"given":"Hatoun","family":"Saeb","sequence":"additional","affiliation":[{"name":"Ministry of Public Health, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Al Thani","sequence":"additional","affiliation":[{"name":"Ministry of Public Health, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3606-9344","authenticated-orcid":false,"given":"Roberto","family":"Bertollini","sequence":"additional","affiliation":[{"name":"Ministry of Public Health, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3719-5142","authenticated-orcid":false,"given":"Hanan F.","family":"Abdul Rahim","sequence":"additional","affiliation":[{"name":"College of Health Sciences, QU Health, Qatar University, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8756-6968","authenticated-orcid":false,"given":"Hiam","family":"Chemaitelly","sequence":"additional","affiliation":[{"name":"World Health Organization Collaborating Centre for Disease Epidemiology Analytics on HIV\/AIDS, Sexually Transmitted Infections, and Viral Hepatitis, Weill Cornell Medicine\u2013Qatar, Cornell University, Qatar Foundation \u2013 Education City, Doha, Qatar"},{"name":"Infectious Disease Epidemiology Group, Weill Cornell Medicine\u2013Qatar, Cornell University, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1583-5484","authenticated-orcid":false,"given":"Patrick","family":"Tang","sequence":"additional","affiliation":[{"name":"Sidra Medicine, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9011-4391","authenticated-orcid":false,"given":"Ali Nizar","family":"Latif","sequence":"additional","affiliation":[{"name":"Hamad Medical Corporation, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saad","family":"Al Kaabi","sequence":"additional","affiliation":[{"name":"Hamad Medical Corporation, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muna A. 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