{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T10:52:11Z","timestamp":1772275931692,"version":"3.50.1"},"reference-count":18,"publisher":"Microbiology Society","issue":"4","license":[{"start":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T00:00:00Z","timestamp":1651017600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"crossref","award":["1316777"],"award-info":[{"award-number":["1316777"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005634","name":"Suomen Hammasl\u00e4\u00e4k\u00e4riseura Apollonia","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005634","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,27]]},"abstract":"<jats:p>Saliva is an alternative sample material to nasopharyngeal swab in SARS-CoV-2 diagnostics. We investigated possible aspects to improve the reliability of SARS-CoV-2 detection from saliva. Saliva was collected from asymptomatic healthy subjects (<jats:italic>n<\/jats:italic>=133) and COVID-19 patients (<jats:italic>n<\/jats:italic>=9). SARS-CoV-2 detection was performed with quantitative reverse-transcriptase PCR (RT-qPCR) with two viral and one host target serving as an internal control. The use of internal control revealed that in the first RT-qPCR run 25\u201330\u200a% of assays failed. The failure is associated with poor RNA quality. When the amount of RNA was cut down to half from the original amount, the performance of RT-qPCR was greatly enhanced (95\u200a% of the assays succeeded). The quality of RNA was not affected by the use of different nucleic acid stabilizing buffers. Our study showed that saliva is suitable material for RT-qPCR based SARS-CoV-2 diagnostics, but the use of internal control is essential to distinguish the true negative samples from failed assays.<\/jats:p>","DOI":"10.1099\/jmm.0.001507","type":"journal-article","created":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T16:17:56Z","timestamp":1649866676000},"source":"Crossref","is-referenced-by-count":2,"title":["Effect of RNA quality to SARS-CoV-2 RT-qPCR detection from saliva"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2048-3420","authenticated-orcid":false,"given":"Susanna","family":"Paju","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, FI-00014 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marika","family":"Tallgren","sequence":"additional","affiliation":[{"name":"PerkinElmer Finland Oy, FI-20101 Turku, 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