{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T03:22:00Z","timestamp":1764904920323,"version":"3.40.5"},"reference-count":15,"publisher":"Microbiology Society","issue":"4","license":[{"start":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T00:00:00Z","timestamp":1651017600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pathology Queensland Study & Education Committee","award":["SERC_6096"],"award-info":[{"award-number":["SERC_6096"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,27]]},"abstract":"\n \n \n Burkholderia pseudomallei<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> is a bipolar Gram-negative bacillus and the causative agent of melioidosis; an infectious disease which commonly presents with bacteraemia.<\/jats:p>\n Data regarding direct from blood culture identification of \n \n B. pseudomallei<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> using the Vitek mass spectrometer (MS) are limited.<\/jats:p>\n The authors aim to assess the safety and sensitivity of the Vitek MS for identification of \n \n B. pseudomallei<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> from spiked positive blood culture samples.<\/jats:p>\n Safety was assessed by determining the ability of the standard MS \u03b1-cyano-4-hydroxycinnamic acid (CHCA) matrix solution to inactivate \n \n B. pseudomallei<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. Organism identification using the manufacturer\u2019s blood culture extraction method was compared to an in-house technique. Additionally, identification following abbreviated agar incubation of blood culture broth was performed.<\/jats:p>\n All 70 MS target spots were inactivated by the matrix solution. The manufacturer\u2019s blood culture extraction method identified 0\/26 (0%) \n \n B. pseudomallei<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> samples. An in-house method using the spun deposit from blood culture broth samples identified 38\/38 (100%) \n \n B. pseudomallei<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> samples. MS analysis of a blood culture broth drop on Chocolate agar following a 6 h incubation identified 30\/32 (94%) samples.<\/jats:p>\n Decreased time to diagnosis of melioidosis bacteraemia is likely to improve patient outcomes. This study adds to the literature with regards to the utility of MALDI-TOF MS identification of \n \n B. pseudomallei<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> both directly from positive blood culture broth and a subsequent 6 h plate incubation. The use of a standard matrix solution inactivates the organism, and use of the spun deposit from a positive blood culture broth is most effective for early identification of \n \n B. pseudomallei<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>.<\/jats:p>","DOI":"10.1099\/jmm.0.001521","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T16:16:21Z","timestamp":1650644181000},"source":"Crossref","is-referenced-by-count":2,"title":["Speed and safety of mass spectrometry for identification of Burkholderia pseudomallei directly from spiked blood cultures"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3548-6963","authenticated-orcid":false,"given":"Ian","family":"Gassiep","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Mater Hospital Brisbane, South Brisbane, Queensland, Australia"},{"name":"University of Queensland Centre for Clinical Research, Royal Brisbane and Woman\u2019s Hospital, Herston, Queensland, Australia"},{"name":"Pathology Queensland, Royal Brisbane & Women\u2019s Hospital, Herston, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2259-5044","authenticated-orcid":false,"given":"Michelle J.","family":"Bauer","sequence":"additional","affiliation":[{"name":"University of Queensland Centre for Clinical Research, Royal Brisbane and Woman\u2019s Hospital, Herston, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2895-0345","authenticated-orcid":false,"given":"Patrick N. A.","family":"Harris","sequence":"additional","affiliation":[{"name":"Pathology Queensland, Royal Brisbane & Women\u2019s Hospital, Herston, Queensland, Australia"},{"name":"University of Queensland Centre for Clinical Research, Royal Brisbane and Woman\u2019s Hospital, Herston, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3948-9272","authenticated-orcid":false,"given":"Robert","family":"Norton","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Queensland, Brisbane, Queensland, Australia"},{"name":"Pathology Queensland, Townsville University Hospital, Townsville, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-19"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2015.8"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.116"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008659"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00081-19"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-021-02276-1"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-017-0173-4"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2015.04.004"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.3201\/eid1407.071501"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00424-21"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01824"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-010-0649-6"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12640"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2018.38.3.235"},{"year":"2019","key":"R15","article-title":"Matrix-assisted laser desorption\/ionisation - time of flight mass spectrometry (MALDI-TOF MS) test: UK Standards for Microbiology Investigations"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001521?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T20:29:01Z","timestamp":1702585741000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001521"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,27]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4,27]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.001521","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"type":"print","value":"0022-2615"},{"type":"electronic","value":"1473-5644"}],"subject":[],"published":{"date-parts":[[2022,4,27]]}}}