{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:08:38Z","timestamp":1777374518641,"version":"3.51.4"},"reference-count":35,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,25]]},"abstract":"\n Introduction.<\/jats:bold> Infectious keratoconjunctivitis is a significant ocular disease found in confined sheep. Little information about the aetiological agents and their antimicrobial susceptibility is available.<\/jats:p>\n \n Gap Statement.<\/jats:bold> There is limited information on the aetiological agents involved in keratoconjunctivitis outbreaks in sheep.<\/jats:p>\n \n Aim.<\/jats:bold> The present research aimed to determine the bacterial aetiological factors involved in an outbreak of infectious keratoconjunctivitis in confined lambs.<\/jats:p>\n \n Methodology.<\/jats:bold> Ocular swabs were collected from 23 randomly selected lambs, which were classified into three groups according to the severity of the lesion: group I (N<\/jats:italic>=6; no ocular involvement), group II (N<\/jats:italic>=8; less severe injuries) and group III (N<\/jats:italic>=9; more severe injuries). Isolation of aerobic bacteria and antimicrobial susceptibility tests were carried out. Molecular detection of \n \n Mollicutes<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> was performed, and positive samples were tested to confirm the presence of the following species: \n \n Mycoplasma conjunctivae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, \n \n Mycoplasma agalactiae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n Mycoplasma mycoides<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> subsp. \n \n capri<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>.<\/jats:p>\n \n Results.<\/jats:bold> \n \n \n Moraxella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> sp. and \n \n Mollicutes<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> were detected in all groups, but we inferred that \n \n Moraxella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> sp. are only significant in the early stages of the disease. \n \n M. conjunctivae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> was detected in all tested groups, while \n \n M. agalactiae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> was detected in samples of group III only. One strain of \n \n Moraxella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> sp. was resistant to erythromycin and showed intermedite resistance to tetracycline.<\/jats:p>\n \n Conclusion.<\/jats:bold> The presence of these species confirms their importance in the aetiology of this disease, and the low resistance profile observed in the studied farm suggested an increased cure success rate.<\/jats:p>","DOI":"10.1099\/jmm.0.001534","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T16:16:16Z","timestamp":1653495376000},"source":"Crossref","is-referenced-by-count":1,"title":["Bacteria associated with infectious keratoconjunctivitis outbreak in confined lambs"],"prefix":"10.1099","volume":"71","author":[{"given":"Rafaela Teixeira","family":"Santos","sequence":"first","affiliation":[{"name":"RuminA\u00e7\u00e3o \u2013 Teaching, Research and Extension in Ruminants, School of Veterinary, Federal University of Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia Carrillo","family":"Gaeta","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"},{"name":"Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brenda Oliveira","family":"Silveira","sequence":"additional","affiliation":[{"name":"RuminA\u00e7\u00e3o \u2013 Teaching, Research and Extension in Ruminants, School of Veterinary, Federal University of Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andressa","family":"Zanette","sequence":"additional","affiliation":[{"name":"RuminA\u00e7\u00e3o \u2013 Teaching, Research and Extension in Ruminants, School of Veterinary, Federal University of Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Riet-Correa","sequence":"additional","affiliation":[{"name":"RuminA\u00e7\u00e3o \u2013 Teaching, Research and Extension in Ruminants, School of Veterinary, Federal University of Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4013-7090","authenticated-orcid":false,"given":"Raquel","family":"Fraga e Silva Raimondo","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"},{"name":"RuminA\u00e7\u00e3o \u2013 Teaching, Research and Extension in Ruminants, School of Veterinary, Federal University of Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","DOI":"10.1201\/b18182","volume-title":"Sheep Medicine","author":"Scott","year":"2015"},{"key":"R2","volume-title":"Sheep and Goat Medicine","author":"Boileau","year":"2012"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2018.11.004"},{"key":"R4","volume-title":"Current Veterinary Therapy-E-Book: Food Animal Practice","author":"Davidson","year":"2008"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1136\/vetreco-2018-000321"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-45-19"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-9-253"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/0921-4488(96)00857-7"},{"key":"R9","volume-title":"Exame oftalmol\u00f3gico e enfermidades oculares em pequenos ruminantes","author":"Gregory","year":"2015"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.4236\/ojvm.2017.710014"},{"key":"R11","volume-title":"Essentials of Veterinary Ophthalmology","author":"Cameron","year":"2001"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.19080\/JDVS.2019.09.555774"},{"key":"R13","first-page":"392","article-title":"Isolation and phenotypic and genotypic identification of Moraxella ovis from clinical cases of ovine keratoconjunctivitis in the state of Mexico","volume":"17","author":"Ortiz-Arana","year":"2019","journal-title":"Ci\u00eancia Animal"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-018-1338-y"},{"key":"R15","doi-asserted-by":"crossref","first-page":"117","DOI":"10.17221\/171\/2019-VETMED","article-title":"Infectious keratoconjunctivitis in\u202fred deer (cervus elaphus) in\u202fpoland \u2013 A case report","volume":"66","author":"Houszka","year":"2021","journal-title":"Vet Med-Czech"},{"key":"R16","volume-title":"Koneman\u2019s Color Atlas and Textbook of Diagnostic Microbiology","author":"Winn","year":"2006"},{"key":"R17","volume-title":"Biochemical Tests for Identification of Medical Bacteria","author":"MacFaddin","year":"2000"},{"key":"R18","article-title":"Performance standards for antimicrobial and dilution susceptibility tests for bacteria isolated from animals: Supplement VET08","year":"2018"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.3.495-503.1990"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.2.655-.1993"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.1999.00218.x"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(95)00058-i"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.6.2.224-230.1999"},{"key":"R24","volume-title":"SPSS Statistics for Windows, Version 27.0","year":"2020"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1935(05)80097-1"},{"key":"R26","volume-title":"Clinical Veterinary Microbiology","author":"Markey","year":"2013"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2008.04.001"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1136\/vropen-2013-000017"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.29155\/vet.57.216.1"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00690-17"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/77.2.399"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731116001622"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1186\/s42523-021-00079-3"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.7589\/2019-04-103"},{"key":"R35","article-title":"No time to wait: securing the future from drug-resistant infections pp.1\u2013 4 Interagency Coordination Group on Antimicrobial Resistance","year":"2017"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001534?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T16:16:27Z","timestamp":1653495387000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,25]]},"references-count":35,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,5,25]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.001534","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,25]]}}}