{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:17:19Z","timestamp":1791343039171,"version":"4.3.1"},"reference-count":26,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,13]]},"abstract":"\n Introduction.<\/jats:bold> The three \n \n Klebsiella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n K. quasipneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> are difficult to distinguish, owing to their similar biochemical properties, and are often confused in medical practice.<\/jats:p>\n \n Gap statement.<\/jats:bold> There is a scarcity of data comparing the clinical characteristics and antimicrobial susceptibility of \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n K. quasipneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. We believe that knowledge of the characteristics of each species will help in their better identification. Further, knowing the antimicrobial susceptibility of the species will help physicians in prescribing an effective treatment course for \n \n Klebsiella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> infections.<\/jats:p>\n \n Aim.<\/jats:bold> This study aimed to determine the clinical characteristics and antimicrobial resistance of \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n K. quasipneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolated from human urine samples.<\/jats:p>\n \n Methodology.<\/jats:bold> This study included 125 \n \n K<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. \n \n pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strains isolated from human urine samples. Multiplex polymerase chain reaction was performed to identify \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n K. quasipneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. We retrospectively investigated the patient background, complications of bacteraemia, antimicrobial susceptibility and extended-spectrum \u03b2-lactamase (ESBL).<\/jats:p>\n \n Results.<\/jats:bold> We identified 84 (67.2\u200a%), 31 (24.8\u200a%) and 10 strains (8\u200a.0%) of \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n K. quasipneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, respectively. There was no difference in patient background and frequency of bacteraemia complications among these species. \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> was significantly less susceptible than \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> to ampicillin\/sulbactam (P<\/jats:italic>=0.03) and piperacillin (P<\/jats:italic><0.01). Furthermore, \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (79.8\u200a%) was less susceptible to trimethoprim\/sulfamethoxazole than \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (96.8\u200a%) and \n \n K. quasipneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (100\u200a%). There were nine ESBL-producing strains (7.2\u200a%), all of which were \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>.<\/jats:p>\n \n Conclusion.<\/jats:bold> There was no difference in patient background and frequency of bacteraemia complications between \n \n K. pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, \n \n K. variicola<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n K. quasipneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolated from urine. The three \n \n Klebsiella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species showed a varying extent of antimicrobial susceptibility and ESBL production, and accurate identification is needed to understand the epidemiology of these species.<\/jats:p>","DOI":"10.1099\/jmm.0.001546","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T10:16:40Z","timestamp":1655201800000},"source":"Crossref","is-referenced-by-count":18,"title":["Clinical characteristics and antimicrobial susceptibility of Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae isolated from human urine in Japan"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8609-4981","authenticated-orcid":false,"given":"Naoki","family":"Watanabe","sequence":"first","affiliation":[{"name":"Hirosaki University, Graduate School of Health Sciences, Hon-cho 66-1, Hirosaki-shi, Aomori, 036-8564, Japan"},{"name":"Department of Clinical Laboratory, Kameda Medical Center, Higashi-cho 929, Kamogawa-shi, Chiba, 296-8602, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5507-8356","authenticated-orcid":false,"given":"Tomohisa","family":"Watari","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Kameda Medical Center, Higashi-cho 929, Kamogawa-shi, Chiba, 296-8602, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihito","family":"Otsuka","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Kameda Medical Center, Higashi-cho 929, Kamogawa-shi, Chiba, 296-8602, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6622-2843","authenticated-orcid":false,"given":"Kazufumi","family":"Yamagata","sequence":"additional","affiliation":[{"name":"Hirosaki University, Graduate School of Health Sciences, Hon-cho 66-1, Hirosaki-shi, Aomori, 036-8564, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miyuki","family":"Fujioka","sequence":"additional","affiliation":[{"name":"Hirosaki University, Graduate School of Health Sciences, Hon-cho 66-1, Hirosaki-shi, Aomori, 036-8564, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00940-06"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-015-0396-6"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2017.01.005"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.03000"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/mSphereDirect.00290-17"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.001143"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4498-x"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/jmmcr.0.000023"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030857"},{"key":"R10","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing: CLSI document M100-S31","year":"2021"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2012.244"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02481-18"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1080\/23744235.2016.1244611"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy077"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.7.2400-2408.2004"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2016.12.013"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70054-4"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01019-15"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01206-19"},{"key":"R20","article-title":"Japan Nosocomial Infections Surveillance (JANIS): Annual Open Report 2020. 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