{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:46:50Z","timestamp":1790365610769,"version":"4.1.0"},"reference-count":18,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T00:00:00Z","timestamp":1658448000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,22]]},"abstract":"\n Introduction.<\/jats:bold> We recently revealed that a significant proportion of clinical methicillin-resistant \n \n Staphylococcus aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (MRSA) isolates are susceptible to pencillins and clavulanic acid (potentiated penicillins), including widely available combinations such as co-amoxiclav. These isolates also showed increased susceptibility to oxacillin on Iso-Sensitest Agar (ISA).<\/jats:p>\n \n Hypothesis\/Gap Statement.<\/jats:bold> The increased susceptibility to oxacillin displayed on ISA by these MRSA isolates may be used to distinguish them from the resistant ones.<\/jats:p>\n \n Aim.<\/jats:bold> We aimed to develop a method to simultaneously screen a \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> clinical isolate for its susceptibility to methicillin and potentiated penicillins.<\/jats:p>\n \n Methodology.<\/jats:bold> A double-disc diffusion method using 10\u2009\u00b5g cefoxitin and 1\u2009\u00b5g oxacillin discs on ISA was developed and tested against a panel of 120 whole genome-sequenced MRSA isolates. The sensitivity of the method was compared with that of previously published genotypic and phenotypic methods. In addition, double-disc diffusion was performed for all isolates on M\u00fcller\u2013Hinton agar (MHA) following the European Committee on Antimicrobial Susceptibility Testing (EUCAST) protocol.<\/jats:p>\n \n Results.<\/jats:bold> All isolates (120\/120) were reconfirmed to be phenotypically MRSA, as indicated by the result of cefoxitin disc diffusion testing. All isolates (40\/40) that had a pencillins and clavulanic acid (Pen\u2013Clav)-resistant genotype were not inhibited by oxacillin, while 77\/80 (96.3\u200a%) isolates that had a Pen\u2013Clav-susceptible genotype were inhibited by oxacillin on ISA. The results also showed that the EUCAST method using MHA correctly identified all isolates as MRSA but failed to distinguish the Pen\u2013Clav-susceptible isolates from the Pen\u2013Clav-resistant isolates.<\/jats:p>\n \n Conclusions.<\/jats:bold> This double-disc diffusion method using ISA could be used to accurately screen for clinical MRSA isolates and determine their susceptibility to Pen\u2013Clav simultaneously, rapidly identifying MRSA infections that might be suitable for treatment with potentiated penicillins.<\/jats:p>","DOI":"10.1099\/jmm.0.001562","type":"journal-article","created":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T19:16:07Z","timestamp":1658517367000},"source":"Crossref","is-referenced-by-count":4,"title":["Simultaneously screening for methicillin-resistant Staphylococcus aureus and its susceptibility to potentiated penicillins"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3882-3585","authenticated-orcid":false,"given":"Xiaoliang","family":"Ba","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University of Cambridge, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire L.","family":"Raisen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Cambridge, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Chao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Environmental Technology College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China"},{"name":"Department of Veterinary Medicine, University of Cambridge, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewan M.","family":"Harrison","sequence":"additional","affiliation":[{"name":"Department of Public Health and Primary Care, University of Cambridge, Cambridge, UK"},{"name":"Department of Medicine, University of Cambridge, Cambridge, UK"},{"name":"Wellcome Sanger Institute, Hinxton, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon J.","family":"Peacock","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cambridge, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5454-1625","authenticated-orcid":false,"given":"Mark A.","family":"Holmes","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Cambridge, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2200"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux007"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0471-0"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.05.024"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00016-17"},{"key":"R6","volume-title":"BSAC Methods for Antimicrobial Susceptibility Testing Version 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