{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:36:15Z","timestamp":1772156175749,"version":"3.50.1"},"reference-count":32,"publisher":"Microbiology Society","issue":"8","funder":[{"name":"MGM Institute of Health Sciences","award":["PVCR:RES:02:2017-18:176"],"award-info":[{"award-number":["PVCR:RES:02:2017-18:176"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,16]]},"abstract":"<jats:p>\n                    <jats:bold>Introduction.<\/jats:bold>\n                    Vaginal colonization of Group B\n                    <jats:italic>\n                      <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5605\" xlink:type=\"simple\">Streptococcus<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    (GBS) is associated with preterm births and neonatal sepsis. Thus routine screening of GBS in prenatal care is recommended.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Hypothesis.<\/jats:bold>\n                    Chromogenic media (carrot broth) aids in specific and rapid detection of GBS.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aim.<\/jats:bold>\n                    To investigate the efficiency of Strep B Carrot Broth for detection of GBS in high vaginal swabs from pregnant women.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods.<\/jats:bold>\n                    In this study 201 vaginal swab samples were collected from pregnant women. Swabs were inoculated in chromogenic media (Strep B Carrot Broth). The positive and negative cultures were inoculated on blood agar and crome agar plates. The colonies were subjected to 16S rRNA sequencing and gene-specific PCR for confirmation. The Christie Atkins Munch Peterson (CAMP) and bile esculin agar (BEA) tests were used for biochemical confirmation. PCR was performed on genomic DNA isolated from uncultured vaginal swabs.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    It was found that 20\/201 (9.9\u200a%) vaginal swab samples were positive in the Strep B Carrot Broth and 17\/20 (85\u200a%) and 19\/20 (95\u200a%) of these samples yielded colonies on blood agar and crome agar, respectively. Of the 181 carrot broth-negative samples, 1 (0.5\u200a%) and 38 (20.9\u200a%) yielded colonies on blood agar and crome agar plates, respectively. However, 16\u2009s rRNA sequencing revealed that none of the 20 carrot broth-positive cultures were GBS and had sequence similarities to\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5526\" xlink:type=\"simple\">Enterococcus faecalis<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    . This was also confirmed by using gene-specific PCR and BEA positivity. Furthermore,\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5526\" xlink:type=\"simple\">E. faecalis<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    was detected by PCR in DNA isolated from 57 uncultured vaginal swabs samples, GBS could only be detected by PCR in four\u2009samples.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion.<\/jats:bold>\n                    Carrot broth-based culture can lead to false-positive detection due to the presence of\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5526\" xlink:type=\"simple\">E. faecalis<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    . Thus GBS-positive results in carrot broth must be confirmed by the other molecular and biochemical tests before making a final diagnosis.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.001577","type":"journal-article","created":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T11:18:56Z","timestamp":1660648736000},"source":"Crossref","is-referenced-by-count":0,"title":["False-positive detection of Group B Streptococcus (GBS) in chromogenic media (Strep B Carrot Broth) due to presence of Enterococcus faecalis in High Vaginal swabs"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7745-2986","authenticated-orcid":false,"given":"Abhishek","family":"Singh","sequence":"first","affiliation":[{"name":"Molecular and Cellular Biology Laboratory, ICMR-National Institute for Research in Reproductive and Child Health, Indian Council of Medical Research (ICMR), Parel, Mumbai, India"},{"name":"Department of Microbiology, MGM Institute of Health Sciences, Kamothe Navi Mumbai, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1372-5700","authenticated-orcid":false,"given":"Atahar","family":"Husein","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology Laboratory, ICMR-National Institute for Research in Reproductive and Child Health, Indian Council of Medical Research (ICMR), Parel, Mumbai, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salomi","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, MGM Institute of Health Sciences, Kalamboli, Navi Mumbai, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2621-6614","authenticated-orcid":false,"given":"Vikas","family":"Ghattargi","sequence":"additional","affiliation":[{"name":"National Centre for Microbial Resource (NCMR), National Centre for Cell Sciences, Pune, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5000-7396","authenticated-orcid":false,"given":"Dhiraj","family":"Dhotre","sequence":"additional","affiliation":[{"name":"National Centre for Microbial Resource (NCMR), National Centre for Cell Sciences, Pune, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5055-5931","authenticated-orcid":false,"given":"Yogesh S.","family":"Shouche","sequence":"additional","affiliation":[{"name":"National Centre for Microbial Resource (NCMR), National Centre for Cell Sciences, Pune, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4083-9815","authenticated-orcid":false,"given":"Stacy","family":"Colaco","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology Laboratory, ICMR-National Institute for Research in Reproductive and Child Health, Indian Council of Medical Research (ICMR), Parel, Mumbai, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9321-415X","authenticated-orcid":false,"given":"Vivek","family":"Abhyankar","sequence":"additional","affiliation":[{"name":"National Centre for Microbial Resource (NCMR), National Centre for Cell Sciences, Pune, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suyash","family":"Patekar","sequence":"additional","affiliation":[{"name":"National Centre for Microbial Resource (NCMR), National Centre for Cell Sciences, Pune, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7571-2836","authenticated-orcid":false,"given":"Karisma","family":"Chhabria","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology Laboratory, ICMR-National Institute for Research in Reproductive and Child Health, Indian Council of Medical Research (ICMR), Parel, Mumbai, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sushil","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, MGM Institute of Health Sciences, Kalamboli, Navi Mumbai, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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