{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:09:24Z","timestamp":1747181364401,"version":"3.40.5"},"reference-count":22,"publisher":"Microbiology Society","issue":"12","license":[{"start":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T00:00:00Z","timestamp":1671408000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,12,19]]},"abstract":"\n Introduction.<\/jats:bold> Invasive meningococcal disease (IMD) caused by \n \n Neisseria meningitidis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> may show temporal and geographical changes in both the epidemiology and the characteristics of the strains involved.<\/jats:p>\n \n Gap statement.<\/jats:bold> A study that examined invasive \n \n N. meningitidis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> causing IMD in Atlantic Canada from 2009 to 2013 was published in 2014. Data from subsequent years have not been described.<\/jats:p>\n \n Aim.<\/jats:bold> This study examined the molecular epidemiology of IMD in four Atlantic Provinces of Canada as well as potential serogroup B (MenB) vaccine coverage.<\/jats:p>\n \n Methods.<\/jats:bold> Individual IMD case isolates recovered from 2014 to 2020 were analysed for serotype and serosubtype antigens as well as by whole-genome sequencing (WGS) for prediction of potential MenB vaccine coverage.<\/jats:p>\n \n Results.<\/jats:bold> Of the 56 IMD isolates, 42, 8, 5 and 1 were MenB, serogroup Y, serogroup W (MenW) and serogroup C, respectively. Geographical differences in the distribution of MenB clones revealed concentration of sequence type (ST)-269 clonal complex (cc) and ST-60 cc in Newfoundland and Labrador, while ST-41\/44\u2009cc (particularly ST-154) was predominantly found in New Brunswick and Nova Scotia. Core genome multi-locus sequence typing (cgMLST) also separated the New Brunswick and Nova Scotia ST-154 isolates into two clusters, with differences in their nhba<\/jats:italic> and penA<\/jats:italic> alleles. Furthermore, cgMLST also separated the ST-269 cc isolates in Atlantic Canada into the ST-1611 and the ST-269\/ST-8924 clusters, with the latter showing high similarity to the ST-269 that first emerged in the Province of Quebec. Genetic Meningococcal Antigen Typing System showed that 54.8\u200a% of MenB were predicted to be covered by the MenB vaccine Bexsero, with a further 38.1\u200a% potentially covered by virtue of the presence of genes that encoded factor H-binding protein variant 1 proteins. Meningococcal deduced vaccine antigen reactivity predicted from WGS data showed that 95.3\u200a% of MenB were covered by Trumenba. Four cases of IMD due to MenW ST-11 cc were also identified, with the first case found in 2018.<\/jats:p>\n \n Conclusions.<\/jats:bold> This study provided evidence concerning the dynamics of \n \n N. meningitidis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strains causing IMD in Atlantic Canada, with both geographical and temporal differences found. MenB vaccine appeared to provide good coverage of MenB IMD, especially towards the predominant strain of ST-154.<\/jats:p>","DOI":"10.1099\/jmm.0.001615","type":"journal-article","created":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T16:21:50Z","timestamp":1671207710000},"source":"Crossref","is-referenced-by-count":1,"title":["Characterization of invasive meningococcal disease case isolates in Atlantic Canada, 2014 to 2020: spatial\u2013temporal variations of clones and predicted meningococcal B vaccine coverage"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4062-4902","authenticated-orcid":false,"given":"Raymond S. 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