{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T01:58:21Z","timestamp":1774749501595,"version":"3.50.1"},"reference-count":34,"publisher":"Microbiology Society","issue":"12","funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"crossref","award":["FRGS\/1\/2019\/SKK06\/UTAR\/03\/2 (8073\/S01)"],"award-info":[{"award-number":["FRGS\/1\/2019\/SKK06\/UTAR\/03\/2 (8073\/S01)"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,12,15]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction<\/jats:bold> Listeriosis, a foodborne infection caused by <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5096\" xlink:type=\"simple\">Listeria monocytogenes<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, could lead to febrile listerial gastroenteritis and a more invasive form which is often associated with a high mortality and hospitalisation rate. Gentamicin, used as an adjunct therapy with ampicillin, remains the treatment of choice for this life-threatening and invasive infection.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap statement<\/jats:bold> Nevertheless, there is little data on gentamicin resistance determinants in <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5096\" xlink:type=\"simple\">L. monocytogenes<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim<\/jats:bold> In this study, we selected and characterised B2b, a gentamicin-resistant mutant derived from <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5096\" xlink:type=\"simple\">L. monocytogenes<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> ATCC 19115 to determine the target(s) of resistance in <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5096\" xlink:type=\"simple\">L. monocytogenes<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> after exposure to gentamicin.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology<\/jats:bold> Whole-genome sequencing was carried out to identify the mutation site(s) and possible mechanism(s) of resistance. The mutant was characterised using antimicrobial susceptibility testing and PCR. For biological verifications, complementation and allelic exchange mutagenesis were carried out.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results<\/jats:bold> We found that the gentamicin resistance in B2b was caused by a 10\u2009bp deletion in <jats:italic>atpG2<\/jats:italic> which encodes a gamma subunit of the ATP synthase in <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5096\" xlink:type=\"simple\">L. monocytogenes<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. Using <jats:italic>atpG2<\/jats:italic> PCR, various other mutations were identified in other gentamicin resistant mutants derived from ATCC 19115. In addition, the mutation from B2b, when introduced into <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10832\" xlink:type=\"simple\">L. ivanovii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, also caused gentamicin resistance in this <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5095\" xlink:type=\"simple\">Listeria<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> species.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion<\/jats:bold> Hence, <jats:italic>atpG2<\/jats:italic> mutations appear to be important determinants of gentamicin resistance not only in <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5096\" xlink:type=\"simple\">L. monocytogenes<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> but possibly also in other <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5095\" xlink:type=\"simple\">Listeria<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> species.<\/jats:p>","DOI":"10.1099\/jmm.0.001618","type":"journal-article","created":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T17:39:32Z","timestamp":1671125972000},"source":"Crossref","is-referenced-by-count":3,"title":["Mutations in atpG2 may confer resistance to gentamicin in Listeria monocytogenes"],"prefix":"10.1099","volume":"71","author":[{"given":"Jamie May Ling","family":"Ng","sequence":"first","affiliation":[{"name":"Centre for Research on Communicable Diseases, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Jalan Sungai Long, Bandar Sungai Long, 43000 Kajang, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1011-762X","authenticated-orcid":false,"given":"Yun Fong","family":"Ngeow","sequence":"additional","affiliation":[{"name":"Centre for Research on Communicable Diseases, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Jalan Sungai Long, Bandar 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