{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:09:24Z","timestamp":1747181364986,"version":"3.40.5"},"reference-count":40,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,10]]},"abstract":"<jats:p><jats:bold>Introduction<\/jats:bold>. Using rapid antigen diagnostic tests (RADTs) in clinical practice has shown excellent specificity but often has diminished sensitivity.<\/jats:p><jats:p><jats:bold>Gap Statement<\/jats:bold>. Local data for evaluating the diagnostic performance of a new fluorescence-based RADT and its influence on the antibiotic prescription rate are not available.<\/jats:p><jats:p><jats:bold>Aim<\/jats:bold>. To evaluate the accuracy of fluorescent immunoassay (FIA)-RADTs for diagnosing group A streptococcal (GAS) pharyngitis among children and its estimated effect as a point of care test (POCT) on the antibiotic prescription rate at the paediatric emergency department.<\/jats:p><jats:p><jats:bold>Methodology<\/jats:bold>. A prospective study was conducted, comprising children 3 to 14\u2009years old presenting with pharyngitis. Throat swab culture and FIA-RADTs were performed on all samples. Conventional PCR was performed on the discordant samples.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold>. A total of 246 children were included in this study. The sensitivity, specificity, and positive and negative predictive values of the FIA-RADT, based on culture results and PCR detection combined, were 95.6, 96.8, 94.6 and 97.4\u200a%, respectively. Antibiotics have been prescribed to 162 (65.9\u200a%) children; however, if FIA-RADTs had been added in the clinical practice as a POCT, only 92 (37.4\u200a%) children would have received antibiotics in total. Additionally, implementation of FIA-RADTs would significantly reduce the antibiotic prescription rate from 48.8 and 60.6\u202f% to 9.5 and 31.9\u202f% among patients with clinical scores of 2 and 3, respectively.<\/jats:p><jats:p><jats:bold>Conclusion<\/jats:bold>. The new FIA-RADT is simple, prompt and reliable. It is helpful in clinical settings and may be used to reduce antibiotic overprescription, especially for children who have a low risk for GAS pharyngitis, according to the clinical score.<\/jats:p>","DOI":"10.1099\/jmm.0.001624","type":"journal-article","created":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T16:19:51Z","timestamp":1676045991000},"source":"Crossref","is-referenced-by-count":0,"title":["Diagnosis of group A streptococcal pharyngitis in the paediatric emergency department using a fluorescence-based RADT: predicted impact on antibiotic prescription"],"prefix":"10.1099","volume":"72","author":[{"given":"Ali J.","family":"Alhaddad","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Medicine, Imam Abdulrahman Bin Faisal University (IAU), Dammam, Saudi 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