{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T10:19:11Z","timestamp":1784715551110,"version":"3.55.0"},"reference-count":27,"publisher":"Microbiology Society","issue":"12","license":[{"start":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T00:00:00Z","timestamp":1671408000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008648","name":"Pfizer Foundation","doi-asserted-by":"crossref","award":["56644459"],"award-info":[{"award-number":["56644459"]}],"id":[{"id":"10.13039\/100008648","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,12,19]]},"abstract":"\n Introduction.<\/jats:bold> Aminoglycosides are used for the treatment of carbapenemase-producing \n \n Klebsiella pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (CPK) infections. 16S rRNA methyltransferases (RMTs) confer resistance to all aminoglycosides and are often cocarried with NDM.<\/jats:p>\n \n Hypothesis\/Gap Statement.<\/jats:bold> There is a dart of studies looking at the aminoglycoside resistance mechanisms for invasive CPK isolates, particularly in OXA-48 endemic settings.<\/jats:p>\n \n Aim.<\/jats:bold> We aimed to determine the prevalence of RMTs and their association with beta lactamases and MLSTs amongst aminoglycoside-resistant CPK bloodstream isolates in an OXA-48 endemic setting.<\/jats:p>\n \n Methodology.<\/jats:bold> CPK isolates (n<\/jats:italic>=181), collected as part of a multicentre cohort study, were tested for amikacin, gentamicin and tobramycin susceptibility using custom-made sensititre plates (GN2XF, Thermo Fisher Scientific). All isolates were previously subjected to whole-genome sequencing. Carbapenemases, RMTs, MLSTs and plasmid incompatibility groups were detected on the assembled genomes.<\/jats:p>\n \n Results.<\/jats:bold> Of the 181 isolates, 109(60\u200a%) were resistant to all three aminoglycosides, and 96 of 109(88\u200a%) aminoglycoside-resistant isolates carried an RMT (85 ArmA, 10 RmtC, 4 RmtF1; three isolates cocarried ArmA and RmtC). Main clonal types associated with ArmA were ST2096 (49\/85, 58\u200a%) and ST14 (24\/85, 28\u200a%), harbouring mainly OXA-232 and OXA-48 +NDM, respectively. RmtC was cocarried with NDM (5\/10) on ST395, and NDM +OXA-48 or NDM +KPC (4\/10) on ST14, ST15 and ST16. All RMT producers also carried CTX-M-15, and the majority cocarried SHV-106, TEM-150 and multiple other antibiotic resistance genes. The majority of the isolates harboured a combination of IncFIB, IncH and IncL\/M type plasmids. Non-NDM producing isolates remained susceptible to ceftazidime-avibactam.<\/jats:p>\n \n Conclusion.<\/jats:bold> Aminoglycoside resistance amongst CPK bloodstream isolates is extremely common and mainly driven by clonal spread of ArmA carried on ST2096 and ST14, associated with OXA-232 and OXA48 +NDM carriage, respectively.<\/jats:p>","DOI":"10.1099\/jmm.0.001629","type":"journal-article","created":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T16:17:01Z","timestamp":1671553021000},"source":"Crossref","is-referenced-by-count":6,"title":["High prevalence of ArmA-16S rRNA methyltransferase among aminoglycoside-resistant Klebsiella pneumoniae bloodstream isolates"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1362-2434","authenticated-orcid":false,"given":"Burcu","family":"Isler","sequence":"first","affiliation":[{"name":"Infection Management Services, Princess Alexandra Hospital, Brisbane, Australia"},{"name":"University of Queensland, Faculty of Medicine, UQ Centre for Clinical Research, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caitlin","family":"Falconer","sequence":"additional","affiliation":[{"name":"University of Queensland, Faculty of Medicine, UQ Centre for Clinical Research, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cansel","family":"Vatansever","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Clinical Microbiology, School of Medicine, Ko\u00e7 University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berna","family":"\u00d6zer","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Clinical Microbiology, School of Medicine, Ko\u00e7 University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcle","family":"\u00c7\u0131nar","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Clinical 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