{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:09:26Z","timestamp":1747181366908,"version":"3.40.5"},"reference-count":36,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T00:00:00Z","timestamp":1678924800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"crossref","award":["C2022405023"],"award-info":[{"award-number":["C2022405023"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100017959","name":"Health Commission of Hebei Province","doi-asserted-by":"crossref","award":["20210702 and 20180843"],"award-info":[{"award-number":["20210702 and 20180843"]}],"id":[{"id":"10.13039\/100017959","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Zhangjiakou Science and Technology Bureau","award":["2121098D"],"award-info":[{"award-number":["2121098D"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,16]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> The <jats:italic>Candida parapsilosis<\/jats:italic> complex can be divided into <jats:italic>C. parapsilosis sensu stricto<\/jats:italic>, <jats:italic>C. orthopsilosis<\/jats:italic>, and <jats:italic>C. metapsilosis<\/jats:italic> subtypes. It is uncommon for drug sensitivity tests to type them.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap Statement.<\/jats:bold> In routine susceptibility reports, drug susceptibility of <jats:italic>C. parapsilosis<\/jats:italic> complex subtypes is lacking.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> The aim of this study is to investigate the antifungal susceptibility and clinical distribution characteristics of the <jats:italic>C. parapsilosis<\/jats:italic> complex subtypes causing deep infection in patients.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Non-repetitive strains of <jats:italic>C. parapsilosis<\/jats:italic> complex isolated from deep infection from 2017 to 2019 were collected. Species-level identification was performed using a matrix-assisted laser desorption\/ionization time-of-flight mass spectrometer and confirmed using <jats:italic>ITS<\/jats:italic> gene sequencing, when necessary. Antifungal susceptibility testing was performed using the Sensititre YeastOne system method.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> A total of 244\u2009cases were included in the study, including 176\u2009males (72.13\u200a%, 60.69\u00b113.43\u2009years) and 68\u2009females (27.87\u200a%, 60.21\u00b110.59\u2009years). The primary diseases were cancer (43.44\u200a%), cardiovascular disease (25.00\u200a%), digestive system diseases, (18.44\u200a%), infection (6.97\u200a%), and nephropathy (6.15\u200a%). Strains were isolated from the bloodstream (63.11\u200a%), central venous catheters (15.16\u200a%), pus (6.56\u200a%), ascites (5.74\u200a%), sterile body fluid (5.33\u200a%), and bronchoalveolar lavage fluid (BALF, 4.09\u200a%). Of the 244 <jats:italic>C. parapsilosis<\/jats:italic> complex strains, 179 (73.26\u200a%) were identified as <jats:italic>C. parapsilosis sensu stricto<\/jats:italic>, 62 (25.41\u200a%) were <jats:italic>C. orthopsilosis<\/jats:italic>, and three (1.23\u200a%) were <jats:italic>C. metapsilosis<\/jats:italic>. Only one <jats:italic>C. parapsilosis sensu stricto<\/jats:italic> strain was resistant to anidulafungin, micafungin, caspofungin, and voriconazole, and it was non-wild-type (NWT) to amphotericin B. Furthermore, six <jats:italic>C. parapsilosis sensu stricto<\/jats:italic> strains were resistant to fluconazole, and one was dose-dependent susceptible. Five <jats:italic>C. parapsilosis sensu stricto<\/jats:italic> strains were NWT to posaconazole. Only one <jats:italic>C. orthopsilosis<\/jats:italic> strain was NWT for anidulafungin, micafungin, caspofungin, fluconazole, voriconazole, amphotericin B, and posaconazole, while the rest of the strains were wild-type.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> \n                  <jats:italic>C. parapsilosis sensu stricto<\/jats:italic> was the main clinical isolate from the <jats:italic>C. parapsilosis<\/jats:italic> complex in our hospital. Most strains were isolated from the bloodstream. The susceptibility rate to commonly used antifungal drugs was more than 96\u200a%. Furthermore, most of the infected patients were elderly male cancer patients.<\/jats:p>","DOI":"10.1099\/jmm.0.001640","type":"journal-article","created":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T13:18:35Z","timestamp":1678972715000},"source":"Crossref","is-referenced-by-count":3,"title":["In vitro susceptibility profiles of Candida parapsilosis species complex subtypes from deep infections to nine antifungal drugs"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0581-2812","authenticated-orcid":false,"given":"Wei","family":"Zhang","sequence":"first","affiliation":[{"name":"Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, PR China, Beijing, 100730"},{"name":"Clinical Laboratory, The First Affiliated Hospital of Hebei North University, No. 12, Changqing Road, Qiaoxi District, Zhangjiakou City, 075000, Hebei Province, PR 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