{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T17:28:22Z","timestamp":1783013302893,"version":"3.54.6"},"reference-count":24,"publisher":"Microbiology Society","issue":"1","funder":[{"name":"Science and Technology Project for People's Livelihood","award":["20377790D"],"award-info":[{"award-number":["20377790D"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,9]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> The prompt and specific diagnosis of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5096\" xlink:type=\"simple\">Listeria monocytogenes<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> meningoencephalitis (LMM) is challenging. Next-generation sequencing (NGS) of cerebrospinal fluid (CSF) is an emerging technique for diagnosing infrequent causative pathogens.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis\/Gap statement.<\/jats:bold> We hypothesized that NGS of CSF is an effective approach for diagnosing LMM.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> To evaluate the effectiveness of NGS, we present five cases of LMM diagnosed using NGS of the CSF.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Between August 2017 and 30 September 2020, we used NGS of the CSF to detect pathogens in patients with clinically suspected central nervous system infections. The clinical characteristics, laboratory tests, imaging findings and NGS results are reviewed.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Five patients were diagnosed with LMM using NGS of the CSF within 2 to 4\u2009days, although the clinical manifestations, medical history and imaging findings varied strikingly. NGS of CSF showed sequence reads corresponding to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5096\" xlink:type=\"simple\">L. monocytogenes<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> species ranging from 118 to 1997\u2009bp, genomic coverage of 0.29\u20135.96\u200a%, relative abundance of 14.83\u201332.16\u200a% and sequencing depth of 1.12 to 1.35. The prompt diagnosis resulted in targeted and effective treatment with the appropriate antibiotics, although two patients with the most severe cerebral parenchymal lesions showed little improvement.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Our results demonstrate the power of NGS of CSF for the prompt diagnosis of LMM. NGS of CSF is an important complementary tool for identifying <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5096\" xlink:type=\"simple\">L. monocytogenes<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>.<\/jats:p>","DOI":"10.1099\/jmm.0.001641","type":"journal-article","created":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T17:18:10Z","timestamp":1673284690000},"source":"Crossref","is-referenced-by-count":9,"title":["Next-generation sequencing for the diagnosis of Listeria monocytogenes meningoencephalitis: a case series of five consecutive patients"],"prefix":"10.1099","volume":"72","author":[{"given":"Lili","family":"Yu","sequence":"first","affiliation":[{"name":"Key Laboratory of Neurology of Hebei Province, Shijiazhuang 050000, Hebei, PR 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Shijiazhuang 050000, Hebei, PR China"},{"name":"Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Neurology of Hebei Province, Shijiazhuang 050000, Hebei, PR China"},{"name":"Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Bu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Neurology of Hebei Province, Shijiazhuang 050000, Hebei, PR China"},{"name":"Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","first-page":"105","article-title":"Listeria monocytogenes 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