{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:49:34Z","timestamp":1787010574003,"version":"build-2736575974"},"reference-count":32,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T00:00:00Z","timestamp":1673222400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004423","name":"World Health Organization","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100004423","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,9]]},"abstract":"<jats:p>\n                    <jats:bold>Introduction.<\/jats:bold>\n                    In 2009, the World Health Organization (WHO) established the Global Invasive Bacterial Vaccine Preventable Disease (IB-VPD) Surveillance Network (GISN) to monitor the global burden and aetiology of bacterial meningitis, pneumonia and sepsis caused by\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3419\" xlink:type=\"simple\">Haemophilus influenzae<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    (Hi),\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1926\" xlink:type=\"simple\">Neisseria meningitidis<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    (Nm) and\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5677\" xlink:type=\"simple\">Streptococcus pneumoniae<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    (Sp).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Hypothesis\/Gap Statement<\/jats:bold>\n                    . The GISN established an external quality assessment (EQA) programme for the characterization of Hi, Nm and Sp by culture and diagnostic PCR.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aim.<\/jats:bold>\n                    To assess the performance of sentinel site laboratories (SSLs), national laboratories (NLs) and regional reference laboratories (RRLs) between 2014 and 2019 in the EQA programme.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methodology.<\/jats:bold>\n                    Test samples consisted of bacterial smears for Gram-staining, viable isolates for identification and serotyping or serogrouping (ST\/SG), plus simulated cerebrospinal fluid (CSF) samples for species detection and ST\/SG by PCR. SSLs and NLs were only required to analyse the slides for Gram staining and identify the species of the live isolates. RRLs, and any SLs and NLs that had the additional laboratory capacity, were also required to ST\/SG the viable isolates and analyse the simulated CSF samples.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    Across the period, 69\u2013112 SS\/NL labs and eight or nine RRLs participated in the EQA exercise. Most participants correctly identified Nm and Sp in Gram-stained smears but were less successful with Hi and other species. SSLs\/NLs identified the Hi, Nm and Sp cultures well and also submitted up to 56\u200a% of Hi, 62\u200a% of Nm and 33\u200a% of Sp optional ST\/SG results each year. There was an increasing trend in the proportion of correct results submitted over the 6\u2009years for Nm and Sp. Some SSLs\/NLs also performed the optional detection and ST\/SG of the three organisms by PCR in simulated CSF from 2015 onwards; 89\u2013100\u200a% of the CSF samples were correctly identified and 76\u201393\u200a% of Hi-, 90\u2013100\u200a% of Nm- and 75\u2013100\u200a% of Sp-positive samples were also correctly ST\/SG across the distributions. The RRLs performed all parts of the EQA to a very high standard, with very few errors across all aspects of the EQA.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion.<\/jats:bold>\n                    The EQA has been an important tool in maintaining high standards of laboratory testing and building of laboratory capacity in the GISN.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.001644","type":"journal-article","created":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T12:19:08Z","timestamp":1673266748000},"source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of the World Health Organization Global Invasive Bacterial Vaccine-Preventable Disease (IB-VPD) Surveillance Network\u2019s Laboratory External Quality Assessment Programme, 2014\u20132019"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9215-0553","authenticated-orcid":false,"given":"David","family":"Litt","sequence":"first","affiliation":[{"name":"World Health Organization Collaborating Centre for Haemophilus influenzae and Streptococcus pneumoniae, United Kingdom Health Security Agency (formerly Public Health England), London, UK"},{"name":"Respiratory and Vaccine Preventable Bacteria Reference Unit, United Kingdom Health Security Agency (formerly Public Health England), London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary P. 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