{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:09:27Z","timestamp":1747181367914,"version":"3.40.5"},"reference-count":43,"publisher":"Microbiology Society","issue":"2","funder":[{"DOI":"10.13039\/100000061","name":"Fogarty International Center","doi-asserted-by":"crossref","award":["FIC\/NIHD43TW009588"],"award-info":[{"award-number":["FIC\/NIHD43TW009588"]}],"id":[{"id":"10.13039\/100000061","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,9]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction<\/jats:bold>. Intraocular tuberculosis (IOTB) is a significant cause of visual morbidity in tuberculosis (TB)-endemic countries. Although <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10886\" xlink:type=\"simple\">Mycobacterium tuberculosis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (<jats:italic>M. tb<\/jats:italic>) has been detected in both the retinal pigment epithelial (RPE) cells and in the intraocular fluid (IOF) in some cases, IOTB is paucibacillary in the vast majority of patients. As a result, <jats:italic>M. tb<\/jats:italic> pathogenesis in the ocular compartment is poorly defined.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis.<\/jats:bold> The transcriptional profile of <jats:italic>M. tb<\/jats:italic> in the ocular compartment will differ from those of <jats:italic>M. tb<\/jats:italic> in environments that represent earlier stages of infection.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim<\/jats:bold>. Our aim is to shed light on the pathogenesis of <jats:italic>M. tb<\/jats:italic> in a clinically relevant but challenging environment to study.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Whole-genome microarray analysis was performed on <jats:italic>M. tb<\/jats:italic> grown in an IOF model (artificial IOF; AIOF) over 6\u2009days against reference log phase bacteria grown in 7H9. Results were compared to published <jats:italic>M. tb<\/jats:italic> transcriptomes in other physiologically relevant environments, e.g. RPE cell line.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> \n                  <jats:italic>M. tb<\/jats:italic> replicates slowly in AIOF. Genes involved in active replication and aerobic respiration as well as lipid metabolism were either downregulated or not differentially expressed. Yet, <jats:italic>M. tb<\/jats:italic> in AIOF downregulated genes of the DosR regulon, indicating the suppression of dormancy, similar to <jats:italic>M. tb<\/jats:italic> in RPE cells. This transcriptional profile is distinct from the active and virulent transcriptomes of <jats:italic>M. tb<\/jats:italic> in alveolar epithelial cells and blood.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> \n                  <jats:italic>M. tb<\/jats:italic> likely acquires a non-invasive and quiescent phenotype, between active infection and dormancy, upon reaching an extrapulmonary niche, i.e. the ocular environment.<\/jats:p>","DOI":"10.1099\/jmm.0.001649","type":"journal-article","created":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T09:19:08Z","timestamp":1676020748000},"source":"Crossref","is-referenced-by-count":0,"title":["Mycobacterium tuberculosis transcriptome in intraocular tuberculosis"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6037-6980","authenticated-orcid":false,"given":"Kamaljit","family":"Kaur","sequence":"first","affiliation":[{"name":"Present address: Public Health 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