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Air samples (<jats:italic>n<\/jats:italic>=19) were collected in 19 restaurant terraces during lunch time. Each air sample was collected using a Coriolis Compact air sampler, followed by RNA extraction and real-time quantitative PCR for the detection of viral RNA. Viral viability was also assessed through RNAse pre-treatment of samples. Only one of the 19 air samples was positive for SARS-CoV-2 RNA, with 7337 gene copies m<jats:sup>\u20133<\/jats:sup> for the genomic region N2, with no viable virus in this sample. The low number of positive samples found in this study is not surprising, as sampling took place in outdoor settings where air circulation is optimal, and aerosols are rapidly dispersed by the air currents. These results are consistent with previous reports stating that transmission of SARS-CoV-2 in outdoor spaces is low, although current evidence shows an association of exposures in settings where drinking and eating is possible on-site with an increased risk in acquiring SARS-CoV-2 infection. Moreover, the minimal infectious dose for SARS-CoV-2 still needs to be determined so that the real risk of infection in different environments can be accurately established.<\/jats:p>","DOI":"10.1099\/jmm.0.001659","type":"journal-article","created":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T16:18:53Z","timestamp":1676045933000},"source":"Crossref","is-referenced-by-count":6,"title":["SARS-CoV-2 in outdoor air following the third wave lockdown release, Portugal, 2021"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8384-0640","authenticated-orcid":false,"given":"Priscilla Gomes da","family":"Silva","sequence":"first","affiliation":[{"name":"Epidemiology Research Unit (EPIUnit), Instituto de Sa\u00fade P\u00fablica da Universidade do Porto, Porto, Portugal"},{"name":"ICBAS \u2013 School of Medicine and Biomedical Sciences, Porto University, Porto, Portugal"},{"name":"LEPABE \u2013 Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Porto, Portugal"},{"name":"ALiCE \u2013 Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal"},{"name":"Laborat\u00f3rio para a Investiga\u00e7\u00e3o Integrativa e Translacional em Sa\u00fade Populacional (ITR), Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria S\u00e3o Jos\u00e9","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Porto, Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia I. 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