{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T18:04:13Z","timestamp":1789927453295,"version":"4.0.1"},"reference-count":16,"publisher":"Microbiology Society","issue":"8","funder":[{"name":"National Key Research and Development Program of China","award":["2021YFC2701800 and 2021YFC2701803"],"award-info":[{"award-number":["2021YFC2701800 and 2021YFC2701803"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["(81861138052"],"award-info":[{"award-number":["(81861138052"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"China Antimicrobial Surveillance Network","award":["2018QD100"],"award-info":[{"award-number":["2018QD100"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,8,8]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Tigecycline is one of the important antibiotics available for treating infection caused by multiple-drug resistant pathogens. However, the conventional AST methods which are commonly used in clinical microbiology laboratories usually lead to false intermediate or resistant results in testing tigecycline susceptibility, and further mislead clinical antimicrobial therapies.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis.<\/jats:bold> The modified Kirby-Bauer disc diffusion (mKB) method was performed based on the traditional standard Kirby-Bauer disc diffusion (sKB) method.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim<\/jats:bold>. To evaluate a modified Kirby-Bauer disc diffusion (mKB) method for tigecycline susceptibility testing, for the purpose of providing accurate tigecycline susceptibility results in clinical practice.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> A total of 4271 nonduplicate clinical strains were isolated from 37 hospitals across China to perform the mKB method, standard Kirby\u2013Bauer disc diffusion (sKB) method, comparing with the reference broth microdilution (BMD) according to the CLSI. Parameters of categorical agreement (CA), minor errors (mE), major errors (ME), and very major errors (VME) were used in this methodological evaluation research.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> BMD testing showed that 91.3\u201398.9\u200a% of the <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2767\" xlink:type=\"simple\">A. baumannii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, <jats:italic>K. pneumoniae<\/jats:italic>, <jats:italic>E. coli<\/jats:italic>, <jats:italic>E. cloacae<\/jats:italic>, <jats:italic>S. marcescens<\/jats:italic>, and <jats:italic>C. freundii<\/jats:italic> strains were susceptible, while 0\u20133.1% strains were resistant to tigecycline. When testing <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2767\" xlink:type=\"simple\">A. baumannii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, mKB demonstrated higher CA than sKB (90.6\u200a% vs 44.8\u200a%) compared to reference BMD. The mE (9.0\u200a% vs 45.2\u200a%), ME (0.5\u200a% vs 10.6\u200a%) and VME (both 0\u200a%) all satisfied the acceptability criteria. mKB also showed higher CA (87.2\u200a% vs 52.0\u200a%) than sKB in comparison with BMD when testing <jats:italic>\n                     <jats:named-content content-type=\"order\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.29303\" xlink:type=\"simple\">Enterobacterales<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (mainly <jats:italic>K. pneumonia<\/jats:italic>). The ME (0.45\u200a% vs 8.1\u200a%) and VME (both 0\u200a%) but not mE (12.4\u200a% vs 40.4\u200a%) met the acceptability criteria.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> The mKB method can test bacterial susceptibility to tigecycline more accurately than sKB. For the tigecycline-intermediate or -resistant strains by sKB method, BMD or mKB method should be used to verify the results and report reliable tigecycline susceptibility results.<\/jats:p>","DOI":"10.1099\/jmm.0.001671","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T13:16:22Z","timestamp":1691500582000},"source":"Crossref","is-referenced-by-count":17,"title":["A modified Kirby-Bauer disc diffusion (mKB) method for accurately testing tigecycline susceptibility: a nation-wide multicenter comparative study"],"prefix":"10.1099","volume":"72","author":[{"given":"Dandan","family":"Yin","sequence":"first","affiliation":[{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, PR China"},{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, PR China"},{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renru","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, PR China"},{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, PR China"},{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, PR China"},{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4493-0619","authenticated-orcid":false,"given":"Fupin","family":"Hu","sequence":"additional","affiliation":[{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, PR China"},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, PR 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