{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:17:24Z","timestamp":1780449444814,"version":"3.54.1"},"reference-count":22,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T00:00:00Z","timestamp":1678924800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangzhou Panyu Administration for industry, commerce and information technology","award":["No. 2020-Z04-007"],"award-info":[{"award-number":["No. 2020-Z04-007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,16]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Early and accurate diagnosis of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.4716\" xlink:type=\"simple\">Mycoplasma pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (<jats:italic>MP<\/jats:italic>) infection of children with pneumonia is at the core of treatment in clinical practice.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap Statement.<\/jats:bold> Serological immunoglobulin M (IgM) tests for <jats:italic>MP<\/jats:italic> infection of children in south China have been rarely described.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> To assess the diagnostic performance and clinical application of serodiagnosis of <jats:italic>MP<\/jats:italic> infection in paediatric pneumonia patients.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Serum samples from 144 children diagnosed with <jats:italic>MP<\/jats:italic> pneumonia were subjected to a particle agglutination (PA)-based IgM assay. Meanwhile, we used an established suspension array as the reference standard method for the detection of <jats:italic>MP<\/jats:italic> DNA in bronchoalveolar lavage fluid (BALF) from all patients to assess the reliability of serological assays.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> When running immunological testing in single serum samples, 80.6\u200a%(79\/98) of cases were diagnosed with <jats:italic>MP<\/jats:italic> infection, whereas only 55 (56.1\u200a%) cases were positive in <jats:italic>MP<\/jats:italic> DNA analysis. Furthermore, single serum tests for IgM during acute <jats:italic>MP<\/jats:italic> infection resulted in 85.5\u200a% (47\/55) sensitivity and 25.6\u200a% (11\/43) specificity. Nevertheless, immunological testing and <jats:italic>MP<\/jats:italic> DNA analysis yielded the same results when paired sera were available for <jats:italic>MP<\/jats:italic> IgM antibody testing.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Paired serological IgM assays are necessary for the determination of an acute <jats:italic>MP<\/jats:italic> infection, whereas single serological IgM testing is unreliable. Moreover, even a short interval of two <jats:italic>MP<\/jats:italic> serological tests works well.<\/jats:p>","DOI":"10.1099\/jmm.0.001673","type":"journal-article","created":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T13:19:19Z","timestamp":1678972759000},"source":"Crossref","is-referenced-by-count":6,"title":["Poor accuracy of single serological IgM tests in children with suspected acute Mycoplasma pneumoniae infection in Guangzhou, China"],"prefix":"10.1099","volume":"72","author":[{"given":"Qiu-Ying","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Guangzhou Panyu Maternal and Children Health Hospital, Guangzhou Panyu District He Xian Memorial Hospital, Guangzhou, 511499, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0782-0688","authenticated-orcid":false,"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Maternal and Child Health Research Institute, Translational Medicine Center, Guangdong Women and Children Hospital, Guangzhou, 511400, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Deng","sequence":"additional","affiliation":[{"name":"Maternal and Child Health Research Institute, Translational Medicine Center, Guangdong Women and Children Hospital, Guangzhou, 511400, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ming","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Guangzhou Panyu Maternal and Children Health Hospital, Guangzhou Panyu District He Xian Memorial Hospital, Guangzhou, 511499, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Lin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Guangzhou Panyu Maternal and Children Health Hospital, Guangzhou Panyu District He Xian Memorial Hospital, Guangzhou, 511499, PR 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