{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T23:48:23Z","timestamp":1768434503695,"version":"3.49.0"},"reference-count":64,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,8]]},"abstract":"\n Introduction.<\/jats:bold> Dextransucrase produced by \n \n Streptococcus mutans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> plays a vital role in the formation of dental caries by synthesizing exopolysaccharides from sucrose, which helps in the attachment of microbes to the tooth surface, causing caries. Exploring antibody production against \n \n S. mutans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> antigens could be an effective method to protect against dental caries.<\/jats:p>\n \n Hypothesis.<\/jats:bold> Dextransucrase antibodies may help in the prevention of caries formation by inhibiting essential cariogenic factors.<\/jats:p>\n \n Aims.<\/jats:bold> The aim of this study was to investigate the effects of dextransucrase antibodies on biofilm formation and certain associated cariogenic factors of \n \n S. mutans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>.<\/jats:p>\n \n Methodology.<\/jats:bold> Dextransucrase was purified from culture of \n \n S. mutans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. The antisera against the enzyme were raised in rabbits. The effect of dextransucrase antibodies on biofilm formation was studied using scanning electron microscopy, fluorescence microscopy and quantitative real-time polymerase chain reaction. The effects of the antibodies on associated cariogenic factors were examined using established methods. The cross-reactivity of antibodies with human lung, liver, heart, thyroid and kidney tissues was evaluated by immunohistochemistry.<\/jats:p>\n \n Results.<\/jats:bold> Our findings showed impaired biofilm formation in \n \n S. mutans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> in the presence of dextransucrase antibodies. Genes associated with biofilm formation such as gtfB, gtfC, brpA, relA, Smu.630<\/jats:italic> and vicK<\/jats:italic> were downregulated (50\u201397\u200a%) by dextransucrase antibodies in \n \n S. mutans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. The adherence of \n \n S. mutans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> to glass surface was reduced by 58\u200a% and hydrophobicity was reduced by 55.2\u200a% in the presence of the antibodies compared to the controls. Immunohistochemistry studies revealed no cross-reactivity of human tissues with dextransucrase antibodies.<\/jats:p>\n \n Conclusions.<\/jats:bold> These findings suggest that antibodies raised against dextransucrase exhibit a profound inhibitory effect on biofilm formation and vital cariogenic factors of \n \n S. mutans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, which supports the contention that dextransucrase could be a promising antigen to study for its anticariogenic potential.<\/jats:p>","DOI":"10.1099\/jmm.0.001696","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T16:16:53Z","timestamp":1686241013000},"source":"Crossref","is-referenced-by-count":4,"title":["Effect of dextransucrase antibodies on biofilm formation and certain cariogenic activities in Streptococcus mutans"],"prefix":"10.1099","volume":"72","author":[{"given":"Shabeer Ahmad","family":"Rather","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Panjab University, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abid","family":"Majeed","sequence":"additional","affiliation":[{"name":"Department of Oral Health Sciences, PGIMER, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakhvinder","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Panjab University, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alka","family":"Bhatia","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine and Biotechnology, PGIMER, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sukesh Chander","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Panjab University, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5650-6308","authenticated-orcid":false,"given":"Akhtar","family":"Mahmood","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Panjab University, Chandigarh, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2012.06.037"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.10.1.227-235.1974"},{"key":"R3","volume-title":"Microbiology for the Healthcare Professional Elsevier Health Sciences","author":"Karin","year":"2013"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.09.129"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040319"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.031591-0"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01493-14"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1139\/W10-095"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.3390\/dj8030071"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.2741\/1305"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2020.1860398"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00024-07"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1186\/s13568-021-01263-z"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2019.02.018"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002623"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.3402\/jom.v6.26189"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510391793"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-016-1602-y"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.5.2004-2011.1995"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-019-10327-x"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.7860\/JCDR\/2013\/5246.3053"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00488"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.219-225.2005"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1128\/iai.20.3.592-599.1978"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.5851\/kosfa.2020.e71"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/0196-6553(89)90199-5"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1177\/00220345810600031701"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-013-1487-3"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517736497"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1177\/00220345940730031301"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-020-02248-0"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.59.110106.132000"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2012.145"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1264\/jsme2.ME19164"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2016.1203496"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.10.2699-2708.2002"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04036.x"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00380-07"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1159\/000016549"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2012.145"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.10.013"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.22038\/ijbms.2008.5239"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01753.x"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1128\/iai.40.1.432-435.1983"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.9.3811-3817.1993"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.19.6383-6390.2004"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.09.129"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.4807849"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1159\/000323376"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10952.x"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00614-06"},{"key":"R53","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47146-0"},{"key":"R54","doi-asserted-by":"publisher","DOI":"10.4103\/0970-4388.155126"},{"key":"R55","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-020-01867-8"},{"key":"R56","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01666-07"},{"key":"R57","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-111"},{"key":"R58","doi-asserted-by":"publisher","DOI":"10.1177\/154411130301400203"},{"key":"R59","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.8.2983-2992.2006"},{"key":"R60","doi-asserted-by":"publisher","DOI":"10.1139\/w02-060"},{"key":"R61","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01395-07"},{"key":"R62","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.12.4064-4076.2005"},{"key":"R63","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.12.1.93-100.2005"},{"key":"R64","first-page":"002","article-title":"Dental caries vaccine availability: challenges for the 21st century","volume":"1","author":"Giasuddin","year":"2017","journal-title":"J Immuno Immunother"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001696?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T16:16:59Z","timestamp":1686241019000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001696"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,8]]},"references-count":64,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6,8]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.001696","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,8]]}}}