{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T11:04:10Z","timestamp":1783854250143,"version":"3.55.0"},"reference-count":37,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:00:00Z","timestamp":1687305600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000576","name":"British Society for Oral and Dental Research","doi-asserted-by":"crossref","award":["Oral and Dental Research Trust"],"award-info":[{"award-number":["Oral and Dental Research Trust"]}],"id":[{"id":"10.13039\/501100000576","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,21]]},"abstract":"\n Introduction.<\/jats:bold> Bacterial pneumonia is a common cause of morbidity and mortality in elderly individuals. While the incidence of edentulism is falling, approximately 19\u200a% of the UK population wear a full or partial removable denture. Despite advances in denture biomaterials, the majority of dentures are fabricated using polymethyl-methacrylate. Growing evidence suggests that colonization of the oral cavity by putative respiratory pathogens predisposes individuals to respiratory infection, by translocation of these microorganisms along the respiratory tract.<\/jats:p>\n \n Hypothesis\/Gap Statement.<\/jats:bold> We hypothesized that denture surfaces provide a susceptible colonization site for putative respiratory pathogens, and thus could increase pneumonia risk in susceptible individuals.<\/jats:p>\n \n Aim.<\/jats:bold> This study aimed to characterize the bacterial community composition of denture-wearers in respiratory health compared with individuals with a confirmed diagnosis of pneumonia.<\/jats:p>\n \n Methodology.<\/jats:bold> This was an analytical cross-sectional study, comparing frail elderly individuals without respiratory infection (n<\/jats:italic>=35) to hospitalized patients with pneumonia (n<\/jats:italic>=26). The primary outcome was the relative abundance of putative respiratory pathogens identified by 16S rRNA metataxonomic sequencing, with quantitative PCR used to identified \n \n Streptococcus pneumoniae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>.<\/jats:p>\n \n Results.<\/jats:bold> There was a statistically significant increase in the overall relative abundance of putative respiratory pathogens (P<\/jats:italic><0.0001), with a greater than 20-fold increase in the bioburden of these microorganisms. In keeping with these findings, there were significant shifts in bacterial community diversity (Chao index, P<\/jats:italic>=0.0003) and richness (Inverse Simpson index P<\/jats:italic><0.0001) in the denture-associated microbiota of pneumonia patients compared with control subjects.<\/jats:p>\n \n Conclusion.<\/jats:bold> Within the limitations of this study, our evidence supports the role of denture acrylic biomaterials as a potential colonization site for putative respiratory pathogens, which may lead to an increased risk of pneumonia in susceptible individuals. These findings support prior observational studies which have found denture-wearers to be at increased risk of respiratory infection. Further research is needed to confirm the sequence of colonization and translocation to examine potential causal relationships.<\/jats:p>","DOI":"10.1099\/jmm.0.001702","type":"journal-article","created":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T13:16:52Z","timestamp":1687353412000},"source":"Crossref","is-referenced-by-count":15,"title":["Compositional shifts within the denture-associated bacteriome in pneumonia \u2013 an analytical cross-sectional study"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3198-6805","authenticated-orcid":false,"given":"Joshua A.","family":"Twigg","sequence":"first","affiliation":[{"name":"Present address: School of Dentistry, University of Leeds, Leeds, UK"},{"name":"School of Dentistry, Cardiff University, Wales, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Smith","sequence":"additional","affiliation":[{"name":"Faculty of Health and Applied Science, University West of England, Bristol, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clotilde","family":"Haury","sequence":"additional","affiliation":[{"name":"School of Dentistry, Cardiff University, Wales, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie J.","family":"Wilson","sequence":"additional","affiliation":[{"name":"School of Dentistry, Cardiff University, Wales, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Lees","sequence":"additional","affiliation":[{"name":"Centre for High Frequency Engineering, Cardiff University, Wales, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Waters","sequence":"additional","affiliation":[{"name":"School of Dentistry, Cardiff University, Wales, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Williams","sequence":"additional","affiliation":[{"name":"School of Dentistry, Cardiff University, Wales, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61766-8"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2009.129502"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1093\/eurpub\/ckp081"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-84882-095-1"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1111\/resp.12663"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-014-2067-1"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01144-2016"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.060010"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2012.6445"},{"key":"R10","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1111\/j.1601-0825.2007.01410a.x","article-title":"Oral biofilms, periodontitis, and pulmonary infections","volume":"13","author":"Paju","year":"2007","journal-title":"Oral Dis"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1177\/154405910808700418"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000212"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrc.2016.07.019"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD008367.pub4"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1754-4505.1999.tb01413.x"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/j.archger.2006.04.004"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1111\/jopr.12342"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1046\/j.1532-5415.2002.50106.x"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/S1532-3382(12)70004-0"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2009.121434"},{"key":"R21","first-page":"357","article-title":"Denture sore mouth: a possible aetiology","volume":"112","author":"Newton","year":"1962","journal-title":"Br Dent J"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.2307\/4582532"},{"key":"R23","article-title":"The European Committee on Antimicrobial Susceptibility Testing Disc diffusion manual v9.0. 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