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Chronic bacterial infection in CF contributes to the gradual destruction of lung tissue, and eventually respiratory failure in this group.<\/jats:p>\n \n Gap Statement.<\/jats:bold> The contribution of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> to cystic fibrosis (CF) lung decline in the presence or absence of \n \n P. aeruginosa<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> is unclear. Defining the molecular and phenotypic characteristics of a range of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> clinical isolates will help further understand its pathogenic capabilities.<\/jats:p>\n \n Aim.<\/jats:bold> Our objective was to use molecular and phenotypic tools to characterise twenty-five clinical \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates collected from mono- and coinfection with \n \n P. aeruginosa<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> from people with CF at the Royal Victoria Infirmary, Newcastle upon Tyne.<\/jats:p>\n \n Methodology.<\/jats:bold> Genomic DNA was extracted and sequenced. Multilocus sequence typing was used to construct phylogeny from the seven housekeeping genes. A pangenome was calculated using Roary, and cluster of Orthologous groups were assigned using eggNOG-mapper which were used to determine differences within core, accessory, and unique genomes. Characterisation of sequence type, clonal complex, agr<\/jats:italic> and spa<\/jats:italic> types was carried out using PubMLST, eBURST, AgrVATE and spaTyper, respectively. Antibiotic resistance was determined using Kirby-Bauer disc diffusion tests. Phenotypic testing of haemolysis was carried out using ovine red blood cell agar plates and mucoid phenotypes visualised using Congo red agar.<\/jats:p>\n \n Results.<\/jats:bold> Clinical strains clustered closely based on agr<\/jats:italic> type, sequence type and clonal complex. COG analysis revealed statistically significant enrichment of COG families between core, accessory and unique pangenome groups. The unique genome was significantly enriched for replication, recombination and repair, and defence mechanisms. The presence of known virulence genes and toxins were high within this group, and unique genes were identified in 11 strains. Strains which were isolated from the same patient all surpassed average nucleotide identity thresholds, however, differed in phenotypic traits. Antimicrobial resistance to macrolides was significantly higher in the coinfection group.<\/jats:p>\n \n Conclusion.<\/jats:bold> There is huge variation in genetic and phenotypic capabilities of \n \n S. aureus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strains. Further studies on how these may differ in relation to other species in the CF lung may give insight into inter-species interactions.<\/jats:p>","DOI":"10.1099\/jmm.0.001703","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T16:17:01Z","timestamp":1686241021000},"source":"Crossref","is-referenced-by-count":3,"title":["Characterisation of key genotypic and phenotypic traits of clinical cystic fibrosis Staphylococcus aureus isolates"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3852-6289","authenticated-orcid":false,"given":"Micaela","family":"Mossop","sequence":"first","affiliation":[{"name":"National Heart and Lung Institute, Imperial College London, London, SW3 6LR, UK"},{"name":"Microbes in Health and Disease Theme, Newcastle University Biosciences Institute, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2561-2858","authenticated-orcid":false,"given":"Luca","family":"Robinson","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute, Imperial College London, London, SW3 6LR, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1543-1634","authenticated-orcid":false,"given":"Jhih-Hang","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, The Alfred Hospital and Central Clinical School, Monash University, Melbourne, Australia"},{"name":"Department of Microbiology, Infection Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2296-2126","authenticated-orcid":false,"given":"Anton Y.","family":"Peleg","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, The Alfred Hospital and Central Clinical School, Monash University, Melbourne, Australia"},{"name":"Department of Microbiology, Infection Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9266-7827","authenticated-orcid":false,"given":"Luke V.","family":"Blakeway","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, The Alfred Hospital and Central Clinical School, Monash University, Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7905-628X","authenticated-orcid":false,"given":"Nenad","family":"Macesic","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, The Alfred Hospital and Central Clinical School, Monash University, Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audrey","family":"Perry","sequence":"additional","affiliation":[{"name":"Department of Health and Life Sciences, Northumbria University, Newcastle upon Tyne. 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