{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:49:32Z","timestamp":1787010572141,"version":"build-2736575974"},"reference-count":24,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T00:00:00Z","timestamp":1688083200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81960382"],"award-info":[{"award-number":["81960382"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008871","name":"Yunnan Provincial Science and Technology Department","doi-asserted-by":"crossref","award":["2019FE001-060"],"award-info":[{"award-number":["2019FE001-060"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100015287","name":"Scholarship for Academic Leader of Yunnan Province","doi-asserted-by":"crossref","award":["D-2017023"],"award-info":[{"award-number":["D-2017023"]}],"id":[{"id":"10.13039\/501100015287","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008871","name":"Yunnan Provincial Science and Technology Department","doi-asserted-by":"crossref","award":["202001AY070001-037"],"award-info":[{"award-number":["202001AY070001-037"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,30]]},"abstract":"\n Introduction.<\/jats:bold>\n The\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -<\/jats:italic>\n positive\n \n \n Enterobacter cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n has led to limited therapeutic options for clinical treatment.\n <\/jats:p>\n \n Hypothesis\/Gap Statement.<\/jats:bold>\n Analysing the antimicrobial resistance and molecular typing of\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -positive\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n is of great significance. Meanwhile, the effect of the\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n gene on the virulence and pathogenicity of\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n remains unclear and should be assessed.\n <\/jats:p>\n \n Aim.<\/jats:bold>\n To understand\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -positive\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n from different perspectives.\n <\/jats:p>\n \n Methodology.<\/jats:bold>\n The PCR was used to screen\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -positive\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n , then, antimicrobial susceptibility tests and multilocus sequence typing (MLST) were performed on them; sixty-nine strains of\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -negative\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n were collected as the controls, 28 pairs of virulence-related genes' carriage and biofilm-forming ability were detected for preliminary evaluation of the virulence phenotype of the strains; to gain insight into the effect of the\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n gene on the virulence and pathogenicity of\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n , the\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -positive\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n T2 (NDM-1), the T2\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n knockout strain (\u0394NDM-1) and ATCC13047 (ST) were studied, compared the motility, anti-serum killing ability, and virulence to cells. Then, the mice intraperitoneal infection model was established, the survival curve, histopathological characteristics, bacterial load in spleen and the contents of cytokines were compared.\n <\/jats:p>\n \n Results.<\/jats:bold>\n (1) Thirty-five\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -positive\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n exhibited multidrug resistance. MLST distinguished 12 STs, ST74 was the most common clonal type (11\/35), followed by ST114 (10\/35). (2) The detection rates of virulence genes\n clpB<\/jats:italic>\n ,\n icmf<\/jats:italic>\n ,\n VasD\/Lip<\/jats:italic>\n and\n acrA<\/jats:italic>\n in the\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -positive\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n were significantly higher than those in\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -negative\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n (\n P<\/jats:italic>\n <0.05), while there was no significant difference in the amount of biofilm formation between two groups. (3) The presence of\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n gene attenuated the motility diameter of\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n , but had no significant effect on their ability to resist serum killing, and the virulence to cells. The survival rate, histopathological changes, bacterial load in spleen and inflammatory cytokines were not significantly affected.\n <\/jats:p>\n \n Conclusions.<\/jats:bold>\n (1) The\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -positive\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n exhibited multidrug resistance, and the MLST typing was mainly ST74 and ST114, with a small-scale clonal spread of the ST114 strain in the hospital NICU ward. (2) The\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n gene did not affect the virulence and pathogenicity of\n \n \n E. cloacae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1099\/jmm.0.001712","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T10:16:15Z","timestamp":1688120175000},"source":"Crossref","is-referenced-by-count":4,"title":["Antimicrobial resistance, molecular characteristics, virulence and pathogenicity of bla\n NDM-1-positive Enterobacter cloacae"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0473-1794","authenticated-orcid":false,"given":"Yan","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory, Yan\u2019an Hospital of Kunming City, Kunming, 650051, PR China"},{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Yunnan Key Laboratory of Laboratory Medicine, Kunming, 650032, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory, Yichang Central People\u2019s Hospital, Yichang Hubei 443003, PR China"},{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Yunnan Key Laboratory of Laboratory Medicine, Kunming, 650032, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Yunnan Key Laboratory of Laboratory Medicine, Kunming, 650032, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruihui","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory, Chuxiong Yi Autonomous Prefecture People\u2019s Hospital, Chuxiong, 675099, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Yunnan Key Laboratory of Laboratory Medicine, Kunming, 650032, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Yunnan Key Laboratory of Laboratory Medicine, Kunming, 650032, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01610"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy660"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00002-19"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjm.2018.04.009"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-65001-4"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens8020049"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05509-11"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux011"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.08.015"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.5812\/jjm.101645"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00115-18"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.61"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2017.0173"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00649-18"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku359"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0192288"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02840-15"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01609"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2020.1780040"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-019-0603-0"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2016.02.019"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku482"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.103620"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2016.08.001"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001712?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T10:16:21Z","timestamp":1688120181000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001712"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,30]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6,30]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.001712","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,30]]}}}