{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T06:42:53Z","timestamp":1773556973530,"version":"3.50.1"},"reference-count":55,"publisher":"Microbiology Society","issue":"7","funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["2019\/19338-8"],"award-info":[{"award-number":["2019\/19338-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["141017\/2021-0"],"award-info":[{"award-number":["141017\/2021-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["304399\/2018-3"],"award-info":[{"award-number":["304399\/2018-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["304803\/2021-9"],"award-info":[{"award-number":["304803\/2021-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","award":["Finance Code 001"],"award-info":[{"award-number":["Finance Code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,18]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold>\n                  <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.11017\" xlink:type=\"simple\">Salmonella enterica<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> serovar Isangi (<jats:italic>S<\/jats:italic>. Isangi) is a rare non-typhoidal serovar, related to invasive nosocomial infections in various countries and to increasing antimicrobial resistance rates.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap statement.<\/jats:bold> Despite existing reports on <jats:italic>S<\/jats:italic>. Isangi, there is a lack of information of specific traits regarding this serovar, which could be improved through genomic analyses.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> Our goals were to characterize the antimicrobial resistance, virulence potential and genomic relatedness of 11 <jats:italic>S<\/jats:italic>. Isangi strains from Brazil in comparison to 185 genomes of global isolates using whole-genome sequencing (WGS) data.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Phenotypic resistance was determined by disc-diffusion. The search for resistance genes, plasmids, prophages, <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3291\" xlink:type=\"simple\">Salmonella<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> pathogenicity islands (SPIs) and virulence genes, plus multi-locus sequence typing (MLST) and core-genome MLST (cgMLST) were performed using WGS.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Brazilian <jats:italic>S<\/jats:italic>. Isangi strains showed phenotypic resistance to nalidixic acid, ciprofloxacin and streptomycin, and harboured antimicrobial resistance [<jats:italic>qnrB19<\/jats:italic>, <jats:italic>aac(6\u2019)-Iaa<\/jats:italic>, <jats:italic>mdsAB<\/jats:italic>] and heavy metal tolerance (<jats:italic>arsD, golST<\/jats:italic>) genes. Col(pHAD28) and IncFII(S) plasmids, virulence genes related to adherence, macrophage induction, magnesium uptake, regulation and type III secretion systems, 12 SPIs and eight prophages were detected. The 185 additional global genomes analysed harboured resistance genes against 11 classes of antimicrobial compounds, 22 types of plasmids, 32 prophages, 14 SPIs, and additional virulence genes related to serum resistance, stress adaptation and toxins. Sequence type (ST)216 was assigned to genomes from Brazil and other countries, while ST335 was the most frequent ST, especially among South African genomes. cgMLST showed that Brazilian genomes were more closely related to genomes from European and African countries, the USA and Taiwan, while the majority of South African genomes were more closely related among each other.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> The presence of <jats:italic>S<\/jats:italic>. Isangi strains from Brazil and different countries showing a close genomic correlation, antimicrobial resistance profiles to drugs used in human therapy and a large number of virulence determinants reinforced the need for stronger initiatives to monitor rare non-typhoidal <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3291\" xlink:type=\"simple\">Salmonella<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> serovars such as <jats:italic>S<\/jats:italic>. Isangi in order to prevent its dissemination among human and non-human sources.<\/jats:p>","DOI":"10.1099\/jmm.0.001736","type":"journal-article","created":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T14:17:44Z","timestamp":1689689864000},"source":"Crossref","is-referenced-by-count":6,"title":["The rare Salmonella enterica serovar Isangi: genomic characterization of the antimicrobial resistance, virulence potential and epidemiology of Brazilian strains in comparison to global isolates"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7506-6181","authenticated-orcid":false,"given":"Felipe Pinheiro","family":"Vilela","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto \u2013 USP, Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Av. do Caf\u00e9, s\/n, 14040-903, Ribeir\u00e3o Preto, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e1lia dos Prazeres","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz \u2013 FIOCRUZ, Av. 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