{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:29:06Z","timestamp":1753885746784,"version":"3.37.3"},"reference-count":43,"publisher":"Microbiology Society","issue":"8","funder":[{"DOI":"10.13039\/501100007338","name":"All-India Institute of Medical Sciences","doi-asserted-by":"crossref","award":["A-COVID-104"],"award-info":[{"award-number":["A-COVID-104"]}],"id":[{"id":"10.13039\/501100007338","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,8,25]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Invasive mucormycosis (IM) is a potentially fatal infection caused by fungi of the order <jats:italic>Mucorales<\/jats:italic>. Histopathology, culture, and radiology are the mainstays of diagnosis, but they are not sufficiently sensitive, resulting in delayed diagnosis and intervention. Recent studies have shown that PCR-based techniques can be a promising way to diagnose IM.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis\/Gap Statement.<\/jats:bold> Early diagnosis of fungal infections using molecular diagnostic techniques can improve patient outcomes, especially in invasive mucormycosis.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> The aim of this study was to evaluate the utility of our in-house mould-specific real time PCR assay (qPCR) in comparison with the commercially available real time PCR (<jats:italic>MucorGenius<\/jats:italic> PCR), for the early diagnosis of mucormycosis in tissue samples from patients with suspicion of invasive mucormycosis (IM). This in-house assay can detect and distinguish three clinically relevant mould species, e.g. <jats:italic>Aspergillus<\/jats:italic> spp., <jats:italic>Mucorales<\/jats:italic> and <jats:italic>Fusarium<\/jats:italic> spp. in a single reaction with only one pair of primers, without the need for sequencing.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> We enrolled 313 tissue samples from 193\u2009patients with suspected IM in this prospective study. All cases were classified using EORTC\/MSGERC guidelines. All samples were tested using traditional methods, in-house qPCR, and MucorGenius PCR.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Using direct microscopy as a gold standard, the overall sensitivity and specificity of in-house qPCR for detection of IM was 92.46% and 80% respectively, while that of the MucorGenius PCR was 66.67% and 90% respectively. However, co-infection of IM and IA adversely affected the performance of MucorGenius PCR in detection of IM.<\/jats:p>\n               <jats:p>The in-house PCR detected <jats:italic>Aspergillus<\/jats:italic> spp. in 14\u2009cases and <jats:italic>Fusarium<\/jats:italic> spp<jats:italic>.<\/jats:italic> in 4\u2009cases which showed clinical and radiological features of fungal sinusitis. The in-house qPCR also performed better in detecting possible cases of IM. This aids early diagnosis and appropriate treatment to improve patient outcomes.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Because the in-house PCR is not only sensitive and specific, but also entirely based on SYBR Green for detection of targets, it is less expensive than probe-based assays and can be used on a regular basis for the diagnosis of IM in resource-constrained settings. It can be used to distinguish between mucormycosis and fungal sinusitis caused by <jats:italic>Aspergillus<\/jats:italic> and <jats:italic>Fusarium<\/jats:italic> in high-risk patients, as well as to accurately detect <jats:italic>Mucorales<\/jats:italic> in fungal co-infection cases.<\/jats:p>","DOI":"10.1099\/jmm.0.001745","type":"journal-article","created":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T10:16:39Z","timestamp":1692958599000},"source":"Crossref","is-referenced-by-count":2,"title":["Utility of in-house and commercial PCR assay in diagnosis of Covid-19 associated mucormycosis in an emergency setting in a tertiary care center"],"prefix":"10.1099","volume":"72","author":[{"given":"Mragnayani","family":"Pandey","sequence":"first","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janya","family":"Sachdev","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renu Kumari","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neha","family":"Sharad","sequence":"additional","affiliation":[{"name":"Department of Lab medicine JPNATC, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anupam","family":"Kanodia","sequence":"additional","affiliation":[{"name":"Department of ENT, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaya","family":"Biswas","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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