{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:10:18Z","timestamp":1791947418672,"version":"4.3.4"},"reference-count":20,"publisher":"Microbiology Society","issue":"9","funder":[{"DOI":"10.13039\/501100014334","name":"JICA Research Institute","doi-asserted-by":"crossref","award":["SATREPS"],"award-info":[{"award-number":["SATREPS"]}],"id":[{"id":"10.13039\/501100014334","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"crossref","award":["22jm0110026h0001"],"award-info":[{"award-number":["22jm0110026h0001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["21K07031"],"award-info":[{"award-number":["21K07031"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100015386","name":"Japan Society for Aeronautical and Space Sciences","doi-asserted-by":"crossref","award":["23K06550"],"award-info":[{"award-number":["23K06550"]}],"id":[{"id":"10.13039\/100015386","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,14]]},"abstract":"<jats:p>\n                  <jats:bold>Background.<\/jats:bold> The spread of <jats:italic>\n                     <jats:named-content content-type=\"family\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3091\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> coproducing carbapenemases, 16S rRNA methylase and mobile colistin resistance proteins (MCRs) has become a serious public health problem worldwide. This study describes two clinical isolates of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10789\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> coharbouring <jats:italic>bla<\/jats:italic>\n                  <jats:sub>IMP-1<\/jats:sub>, <jats:italic>armA<\/jats:italic> and <jats:italic>mcr-10<\/jats:italic>.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methods.<\/jats:bold> Two clinical isolates of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10789\" xlink:type=\"simple\">K. pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> resistant to carbapenems and aminoglycosides were obtained from two patients at a hospital in Myanmar. Their minimum inhibitory concentrations (MICs) were determined by broth microdilution methods. The whole-genome sequences were determined by MiSeq and MinION methods. Drug-resistant factors and their genomic environments were determined.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> The two <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10789\" xlink:type=\"simple\">K. pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> isolates showed MICs of \u22654 and \u22651024\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup> for carbapenems and aminoglycosides, respectively. Two <jats:italic>K. pneumonaie<\/jats:italic> harbouring <jats:italic>mcr-10<\/jats:italic> were susceptible to colistin, with MICs of \u22640.015\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup> using cation-adjusted Mueller\u2013Hinton broth, but those for colistin were significantly higher (0.5 and 4\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup>) using brain heart infusion medium. Whole-genome analysis revealed that these isolates coharboured <jats:italic>bla<\/jats:italic>\n                  <jats:sub>NDM-1<\/jats:sub>, <jats:italic>armA<\/jats:italic> and <jats:italic>mcr-10<\/jats:italic>. These two isolates showed low MICs of 0.25\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup> for colistin. Genome analysis revealed that both <jats:italic>bla<\/jats:italic>\n                  <jats:sub>NDM-1<\/jats:sub> and <jats:italic>armA<\/jats:italic> were located on IncFIIs plasmids of similar size (81\u2009kb). The <jats:italic>mcr-10<\/jats:italic> was located on IncM2 plasmids of sizes 220 or 313\u2009kb in each isolate. These two isolates did not possess a <jats:italic>qseBC<\/jats:italic> gene encoding a two-component system, which is thought to regulate the expression of <jats:italic>mcr<\/jats:italic> genes.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> This is the first report of isolates of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10789\" xlink:type=\"simple\">K. pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> coharbouring <jats:italic>bla<\/jats:italic>\n                  <jats:sub>NDM-1<\/jats:sub>, <jats:italic>armA<\/jats:italic> and <jats:italic>mcr-10<\/jats:italic> obtained in Myanmar.<\/jats:p>","DOI":"10.1099\/jmm.0.001750","type":"journal-article","created":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T14:43:16Z","timestamp":1694702596000},"source":"Crossref","is-referenced-by-count":4,"title":["Klebsiella pneumoniae co-harbouring bla\n            NDM-1\n            , armA and mcr-10 isolated from blood samples in Myanmar"],"prefix":"10.1099","volume":"72","author":[{"given":"Tatsuya","family":"Tada","sequence":"first","affiliation":[{"name":"Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Oshiro","sequence":"additional","affiliation":[{"name":"Juntendo Advanced Research Institute for Health Science, Juntendo University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Microbiome Research, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Tohya","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomomi","family":"Hishinuma","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Thi","family":"Htoon","sequence":"additional","affiliation":[{"name":"National Health Laboratory, Yangon, Myanmar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Htay Htay","family":"Tin","sequence":"additional","affiliation":[{"name":"National Health Laboratory, Yangon, Myanmar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruo","family":"Kirikae","sequence":"additional","affiliation":[{"name":"Department of 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