{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T02:41:11Z","timestamp":1789094471837,"version":"build-2803163510"},"reference-count":34,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,6]]},"abstract":"<jats:p>Species of the genus <jats:italic>Candida,<\/jats:italic> characterized as commensals of the human microbiota, are opportunistic pathogens capable of generating various types of infections with high associated costs. Considering the limited pharmacological arsenal and the emergence of antifungal-resistant strains, the repositioning of drugs is a strategy used to search for new therapeutic alternatives, in which minocycline and doxycycline have been evaluated as potential candidates. Thus, the objective was to evaluate the <jats:italic>in vitro<\/jats:italic> antifungal activity of two tetracyclines, minocycline and doxycycline, and their possible mechanism of action against fluconazole-resistant strains of <jats:italic>Candida<\/jats:italic> spp. The sensitivity test for antimicrobials was performed using the broth microdilution technique, and the pharmacological interaction with fluconazole was also analysed using the checkerboard method. To analyse the possible mechanisms of action, flow cytometry assays were performed. The minimum inhibitory concentration obtained was 4\u2013427\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup> for minocycline and 128\u2013512\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup> for doxycycline, and mostly indifferent and additive interactions with fluconazole were observed. These tetracyclines were found to promote cellular alterations that generated death by apoptosis, with concentration-dependent reactive oxygen species production and reduced cell viability. Therefore, minocycline and doxycycline present themselves as promising study molecules against <jats:italic>Candida<\/jats:italic> spp.<\/jats:p>","DOI":"10.1099\/jmm.0.001759","type":"journal-article","created":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T13:17:06Z","timestamp":1696598226000},"source":"Crossref","is-referenced-by-count":3,"title":["Analysis of possible pathways on the mechanism of action of minocycline and doxycycline against strains of Candida spp. resistant to fluconazole"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4964-351X","authenticated-orcid":false,"given":"Cec\u00edlia Rocha","family":"da Silva","sequence":"first","affiliation":[{"name":"Drug Research & Development Center, Federal University of Cear\u00e1, Fortaleza, CE, Brazil"},{"name":"School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Cear\u00e1, Fortaleza, CE, 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