{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T02:27:27Z","timestamp":1789698447250,"version":"4.0.1"},"reference-count":35,"publisher":"Microbiology Society","issue":"12","license":[{"start":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T00:00:00Z","timestamp":1701820800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Young Talent Development Plan of Changzhou Health Commission","award":["CZQM2020113"],"award-info":[{"award-number":["CZQM2020113"]}]},{"DOI":"10.13039\/501100017538","name":"Zhejiang Provincial Ten Thousand Plan for Young Top Talents","doi-asserted-by":"crossref","award":["CZQM2022023"],"award-info":[{"award-number":["CZQM2022023"]}],"id":[{"id":"10.13039\/501100017538","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Key Laboratory Project of Changzhou City","award":["CM20223016"],"award-info":[{"award-number":["CM20223016"]}]},{"name":"Leading Talent of Changzhou \u201cThe 14th Five-Year Plan\u201d High-Level Health Talents Training Project","award":["2022CZLJ025"],"award-info":[{"award-number":["2022CZLJ025"]}]},{"name":"Changzhou Science and Technology Plan Project","award":["CE20225041"],"award-info":[{"award-number":["CE20225041"]}]},{"name":"Changzhou Science and Technology Plan Project","award":["CJ20220114"],"award-info":[{"award-number":["CJ20220114"]}]},{"DOI":"10.13039\/501100013058","name":"Key Research and Development Project of Jiangsu Province","doi-asserted-by":"crossref","award":["BE2023694"],"award-info":[{"award-number":["BE2023694"]}],"id":[{"id":"10.13039\/501100013058","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,6]]},"abstract":"\n Introduction<\/jats:bold>. \n \n Chlamydia psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (\n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>) is a zoonotic infection, that causes psittacosis (parrot fever) in humans, leading to severe clinical manifestations, including severe pneumonia, adult respiratory distress syndrome, and, in rare cases, death.<\/jats:p>\n \n Gap Statement<\/jats:bold>. Rapid, sensitive and specific detection of \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> facilitates timely diagnosis and treatment of patients.<\/jats:p>\n \n Aim<\/jats:bold>. This study aimed to engineer the LAMP-CRISPR\/Cas12b platform for \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> detection.<\/jats:p>\n \n Methodology<\/jats:bold>. The loop-mediated isothermal amplification (LAMP) technique and clustered regularly interspaced short palindromic repeats-CRISPR associated protein 12b (CRISPR-Cas12b) assay were combined to establish two-step and one-tube LAMP-CRISPR\/Cas12b reaction systems, respectively, for rapidly detecting \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>.<\/jats:p>\n \n Results<\/jats:bold>. The two-step and one-tube LAMP-CRISPR\/Cas12b assay could complete detection within 1\u2009h. No cross-reactivity was observed from non-\n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> templates with specific LAMP amplification primers and single-guide RNA (sgRNA) targeting the highly conserved short fragment CPSIT_0429 gene of \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. The detection limits of the two-step and one-tube LAMP-CRISPR\/Cas12b reaction were 102<\/jats:sup> aM and 103<\/jats:sup> aM, respectively. The results were consistent with qPCR for nucleic acid detection in 160 clinical samples, including 80 suspected \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> samples, kept in the laboratory.<\/jats:p>\n \n Conclusions<\/jats:bold>. The LAMP-CRISPR\/Cas12b assay developed in this study provides a sensitive and specific method for rapidly detecting \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and offers technical support for its rapid diagnosis.<\/jats:p>","DOI":"10.1099\/jmm.0.001781","type":"journal-article","created":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T13:17:43Z","timestamp":1701868663000},"source":"Crossref","is-referenced-by-count":17,"title":["Engineering of the LAMP-CRISPR\/Cas12b platform for Chlamydia psittaci detection"],"prefix":"10.1099","volume":"72","author":[{"given":"Rong","family":"Wang","sequence":"first","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xujian","family":"Mao","sequence":"additional","affiliation":[{"name":"Pathogen Inspection Center, Changzhou Center for Disease Prevention and Control, Changzhou, Jiangsu 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