{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:33:14Z","timestamp":1762432394600,"version":"3.40.5"},"reference-count":25,"publisher":"Microbiology Society","issue":"1","funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["21K08506"],"award-info":[{"award-number":["21K08506"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,8]]},"abstract":"<jats:p>\n                  <jats:bold>Background.<\/jats:bold>\n                  <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5677\" xlink:type=\"simple\">Streptococcus pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> is a major causative bacteria of pneumonia and invasive pneumococcal disease (IPD); however, the mechanisms underlying its severity and invasion remain to be defined. Pneumococcal colonies exhibit opaque and transparent opacity phase variations, which have been associated with invasive infections and nasal colonization, respectively, in animal studies. This study evaluated the relationship between the opacity of pneumococcal colonies and the clinical presentation of pneumococcal pneumonia.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methods.<\/jats:bold> This retrospective study included adult patients hospitalized with pneumococcal pneumonia between 2012 and 2019 at four tertiary medical institutions. Pneumococcal strains from lower respiratory tract specimens were determined for their serotypes and microscopic colony opacity, and the association between the opacity phase and the severity of pneumonia was evaluated. Serotypes 3 and 37 with mucoid colony phenotypes were excluded from the study because their colony morphologies were clearly different.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> A total of 92\u2009patients were included. Most patients were older adults (median age: 72\u2009years) and males (67\u200a%), and 59\u200a% had community-acquired pneumonia. Of the 92\u2009patients, 41 (45\u200a%), 12 (13\u200a%), and 39 (42\u200a%) patients had opaque, transparent, and mixed variants in their pneumococcal colony, respectively. The opaque and non-opaque pneumococcal variants had no statistically significant difference in patient backgrounds. Although the pneumonia severity index score did not differ between the opaque and non-opaque groups, the rate of bacteremia was significantly higher in the opaque group than in the non-opaque group. Serotype distribution was similar between the groups.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions.<\/jats:bold> Opaque pneumococcal variants may cause pneumonia and invasive diseases in humans. This study could help elucidate IPD, and opacity assessment may serve as a predictor for IPD.<\/jats:p>","DOI":"10.1099\/jmm.0.001784","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T13:17:23Z","timestamp":1704719843000},"source":"Crossref","is-referenced-by-count":1,"title":["Clinical features relating to pneumococcal colony phase variation in hospitalized adults with pneumonia"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4127-2734","authenticated-orcid":false,"given":"Shuhei","family":"Ideguchi","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine, Nagasaki University Hospital, Nagasaki, Japan"},{"name":"First Department of Internal Medicine, Division of Infectious, Respiratory, and Digestive Medicine, University of the Ryukyus Graduate School of Medicine, Okinawa, 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