{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T21:23:28Z","timestamp":1773005008661,"version":"3.50.1"},"reference-count":75,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T00:00:00Z","timestamp":1705536000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical Research Council","award":["MR\/R026262\/1"],"award-info":[{"award-number":["MR\/R026262\/1"]}]},{"name":"Ministry of Science and Technology","award":["NDT.79.GB\/20"],"award-info":[{"award-number":["NDT.79.GB\/20"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,18]]},"abstract":"\n Introduction.<\/jats:bold>\n \n \n Helicobacter pylori<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> infection is a major global health concern, linked to the development of various gastrointestinal diseases, including gastric cancer. To study the pathogenesis of \n \n H. pylori<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and develop effective intervention strategies, appropriate animal pathogen models that closely mimic human infection are essential.<\/jats:p>\n \n Gap statement.<\/jats:bold> This study focuses on the understudied hpEastAsia genotype in Southeast Asia, a region marked by a high \n \n H. pylori<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> infection rate. No mouse-adapted model strains has been reported previously. Moreover, it recognizes the urgent requirement for vaccines in developing countries, where overuse of antimicrobials is fuelling the emergence of resistance.<\/jats:p>\n \n Aim.<\/jats:bold> This study aims to establish a novel mouse-adapted \n \n H. pylori<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> model specific to the hpEastAsia genotype prevalent in Southeast Asia, focusing on comparative genomic and histopathological analysis of pathogens coupled with vaccine preclinical studies.<\/jats:p>\n \n Methodology.<\/jats:bold> We collected and sequenced the whole genome of clinical strains of \n \n H. pylori<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> from infected patients in Vietnam and performed comparative genomic analyses of \n \n H. pylori<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strains in Southeast Asia. In parallel, we conducted preclinical studies to assess the pathogenicity of the mouse-adapted \n \n H. pylori<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strain and the protective effect of a new spore-vectored vaccine candidate on male Mlac:ICR mice and the host immune response in a female C57BL\/6\u2009mouse model.<\/jats:p>\n \n Results.<\/jats:bold> Genome sequencing and comparison revealed unique and common genetic signatures, antimicrobial resistance genes and virulence factors in strains HP22 and HP34; and supported clarithromycin-resistant HP34 as a representation of the hpEastAsia genotype in Vietnam and Southeast Asia. HP34-infected mice exhibited gastric inflammation, epithelial erosion and dysplastic changes that closely resembled the pathology observed in human \n \n H. pylori<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> infection. Furthermore, comprehensive immunological characterization demonstrated a robust host immune response, including both mucosal and systemic immune responses. Oral vaccination with candidate vaccine formulations elicited a significant reduction in bacterial colonization in the model.<\/jats:p>\n \n Conclusion.<\/jats:bold> Our findings demonstrate the successful development of a novel mouse-adapted \n \n H. pylori<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> model for the hpEastAsia genotype in Vietnam and Southeast Asia. Our research highlights the distinctive genotype and pathogenicity of clinical \n \n H. pylori<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strains in the region, laying the foundation for targeted interventions to address this global health burden.<\/jats:p>","DOI":"10.1099\/jmm.0.001786","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T11:40:45Z","timestamp":1705578045000},"source":"Crossref","is-referenced-by-count":2,"title":["Genomic and vaccine preclinical studies reveal a novel mouse-adapted Helicobacter pylori model for the hpEastAsia genotype in Southeast Asia"],"prefix":"10.1099","volume":"73","author":[{"given":"Thi Kim Cuc","family":"Nguyen","sequence":"first","affiliation":[{"name":"Institute of Biotechnology and Environment, Nha Trang University, 2 Nguyen Dinh Chieu Street, Khanh Hoa, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hoang Dang 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