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In Brazil, <jats:italic>S<\/jats:italic>. 1,4, [5],12:i:- was identified as one of the most prevalent serovars in S\u00e3o Paulo State during 2004\u20132020.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap Statement.<\/jats:bold> However, few studies have characterized this serovar in Brazil.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> This study aimed to determine the antimicrobial resistance profiles of <jats:italic>S<\/jats:italic>. 1,4, [5],12:i:- strains isolated from different sources in Southeast Brazil and compare their genetic diversity.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> We analysed 113\u2009<jats:italic>S<\/jats:italic>. 1,4, [5],12:i:- strains isolated from humans (<jats:italic>n<\/jats:italic>=99), animals (<jats:italic>n<\/jats:italic>=7), food (<jats:italic>n<\/jats:italic>=5) and the environment (<jats:italic>n<\/jats:italic>=2) between 1983 and 2020. Susceptibility testing against 13 antimicrobials was performed using the disc diffusion method for all the strains. Plasmid resistance genes and mutations in the quinolone resistance-determining regions were identified in phenotypically fluoroquinolone-resistant strains. Molecular typing was performed using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) for all strains and multilocus sequence typing (MLST) for 40 selected strains.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Of the 113 strains, 54.87\u200a% were resistant to at least one antimicrobial. The highest resistance rates were observed against ampicillin (51.33\u200a%), nalidixic acid (39.82\u200a%) and tetracycline (38.05\u200a%). Additionally, 39 (34.51\u200a%) strains were classified as multidrug-resistant (MDR). Nine fluoroquinolone-resistant strains exhibited the <jats:italic>gyrA<\/jats:italic> mutation (Ser96\u2192Tyr96) and contained the <jats:italic>qnrB<\/jats:italic> gene. The 113 strains were grouped into two clusters using ERIC-PCR, and most of strains were present in one cluster, with a genetic similarity of \u226580\u200a%. Finally, 40 strains were typed as ST19 using MLST.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> The prevalence of MDR strains is alarming because antimicrobial treatment against these strains may lead to therapeutic failure. Furthermore, the ERIC-PCR and MLST results suggested that most strains belonged to one main cluster. Thus, a prevalent subtype of <jats:italic>Salmonella<\/jats:italic> 1,4, [5],12:i:- strains has probably been circulating among different sources in S\u00e3o Paulo, Brazil, over decades.<\/jats:p>","DOI":"10.1099\/jmm.0.001792","type":"journal-article","created":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T12:17:22Z","timestamp":1708431442000},"source":"Crossref","is-referenced-by-count":2,"title":["High levels of multidrug-resistant isolates of genetically similar Salmonella 1,4, [5],12:I:- from Brazil between 1983 and 2020"],"prefix":"10.1099","volume":"73","author":[{"given":"Giovana do Nascimento","family":"Pereira","sequence":"first","affiliation":[{"name":"Universidade de S\u00e3o Paulo (USP), Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto (FCFRP), Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas (DACTB), Ribeir\u00e3o Preto, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda 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