{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:09:57Z","timestamp":1777612197719,"version":"3.51.4"},"reference-count":19,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,9]]},"abstract":"<jats:p>Sexually transmitted infections (STI) are a public health problem. Real-time PCR assays are the most sensitive test for screening and diagnosis of these infections. The aim of this study was to evaluate a new CT\/NG\/TV\/MG Real-Time PCR (RT-PCR) kit (Vircell) for the detection of <jats:italic>Chamydia trachomatis<\/jats:italic>, <jats:italic>Neisseria gonorrhoeae<\/jats:italic>, <jats:italic>Mycoplasma genitalium<\/jats:italic> and <jats:italic>Trichomonas vaginalis<\/jats:italic> for the diagnosis of sexual transmitted infections using the Allplex STI Essential Assay (Seegene) as the reference\u2019s method. A total of 497\u2009samples from different anatomical sites (endocervical, urethral, rectal, pharyngeal and urine) were analysed from October 2022 to February 2023. A total of 108 (21.73\u200a%) and 106 (21.33\u200a%) positive samples were found for any of the assays used. The most commonly detected pathogen was <jats:italic>N. gonorrhoeae<\/jats:italic> (52\u2009samples; 10.46\u200a%), and the least commonly detected was <jats:italic>T. vaginalis<\/jats:italic> (three\u2009samples; 0.60\u200a%). The anatomical site with the highest prevalence of micro-organisms was a non-urogenital site, the pharynx (26 positive samples; 5.23\u200a%). Using the Allplex STI Essential Assay (Seegene) as the reference method, the diagnosis performance showed that the average specificity of CT\/NG\/TV\/MG RT-PCR Kit (Vircell) was 99.84\u200a% and the sensitivity was 99.53\u200a%. The overall concordance was k=0.98 (CI95\u200a%; 0.96\u20131). In conclusion, the CT\/NG\/TV\/MG RT-PCR Kit (Vircell) assay shows a good sensitivity and specificity and constitutes a promising and additional alternative to routine procedures for distinct types of clinical specimen in diagnosis STI.<\/jats:p>","DOI":"10.1099\/jmm.0.001797","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T11:19:40Z","timestamp":1712661580000},"source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of a new CT\/NG\/TV\/MG Real-Time PCR Kit (Vircell) versus the Allplex STI Essential Assay (Seegene) for the diagnosis of sexually transmitted infections"],"prefix":"10.1099","volume":"73","author":[{"given":"Mar\u00eda","family":"Aguilera-Franco","sequence":"first","affiliation":[{"name":"Microbiology Service, Hospital Universitario Virgen de las Nieves, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Tarri\u00f1o-Le\u00f3n","sequence":"additional","affiliation":[{"name":"Microbiology Service, Hospital Universitario Virgen de las Nieves, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MJ","family":"Olivares-Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Clinical Analysis Service, Hospital Universitario Virgen de las Nieves, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Espadafor","sequence":"additional","affiliation":[{"name":"Dermatology Service, Centro de ETS, Hospital Universitario Virgen de las Nieves, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Rodr\u00edguez-Granger","sequence":"additional","affiliation":[{"name":"Microbiology Service, Hospital Universitario Virgen de las Nieves, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Antonio","family":"Reguera","sequence":"additional","affiliation":[{"name":"Microbiology Service, Hospital Universitario Virgen de las Nieves, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Cobo","sequence":"additional","affiliation":[{"name":"Microbiology Service, Hospital Universitario Virgen de las Nieves, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Sampedro","sequence":"additional","affiliation":[{"name":"Microbiology Service, Hospital Universitario Virgen de las Nieves, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Mar\u00eda","family":"Navarro","sequence":"additional","affiliation":[{"name":"Microbiology Service, Hospital Universitario Virgen de las Nieves, Granada, 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