{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T10:49:26Z","timestamp":1781174966477,"version":"3.54.1"},"reference-count":30,"publisher":"Microbiology Society","issue":"2","funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"crossref","award":["ECR\/2018\/002478"],"award-info":[{"award-number":["ECR\/2018\/002478"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,5]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> The rise of multi-drug-resistant bacteria poses a global threat. In 2017, the World Health Organization identified 12 antibiotic-resistant \u2018priority pathogens\u2019, including Enterobacteriaceae, highlighting the menace of Gram-negative bacteria. Diarrhoeagenic <jats:italic>Escherichia coli<\/jats:italic> (DEC)-induced diarrhoea is particularly problematic for travellers and infants. In contrast to other antibiotic alternatives, passive immunotherapy is showing promise by providing immediate and precise protection. However, mammalian-sourced antibodies are costly, hindering large-scale production. Egg-laying chicken-derived IgY antibodies present a cost-effective, high-yield solution, revolutionizing antibody-based therapeutics compared to mammalian IgG.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis\/Gap Statement.<\/jats:bold> This study hypothesized that developing anti-DEC-IgY could combat DEC infections effectively.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> The primary aim was to develop anti-DEC-IgY and assess its potential in DEC-induced diarrhoeal management.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Method.<\/jats:bold> Chickens were immunized with DEC antigens to induce an immune response. IgY antibodies were extracted from immune eggs and purified using ion-exchange column chromatography. Anti-DEC-IgY\u2019s ability to inhibit DEC growth was evaluated through growth inhibition assays. Anti-DEC-IgY\u2019s capacity to prevent <jats:italic>E. coli<\/jats:italic> adhesion was assessed using mice intestinal mucosa. <jats:italic>In vivo<\/jats:italic> experiments measured pathogen colonization reduction and infection severity reduction. <jats:italic>P<\/jats:italic> values were calculated to confirm statistical significance.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Result.<\/jats:bold> The antibacterial efficacy of anti-DEC-IgY by growth inhibition assay demonstrated that 25\u2009mg\u2009ml<jats:sup>\u22121<\/jats:sup> of IgY could inhibit the DEC growth. The anti-adherence-property was tested using mice intestinal mucosa and found that anti-DEC-IgY could prevent the <jats:italic>E. coli<\/jats:italic> adhesion. <jats:italic>In vivo<\/jats:italic> results suggest that 12\u2009mg\u2009ml<jats:sup>\u22121<\/jats:sup> of IgY will reduce the pathogen colonization in intestine and reduce the severity of the infection. The <jats:italic>P<\/jats:italic> values between the experimental groups confirm the statistical significance of the findings.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> The study findings suggest that IgY-based passive immunotherapy could be a potential strategy for managing the risks associated with antibiotic-resistant bacterial infections. Additionally, this study paves the way for the development of IgY-related research and applications in India.<\/jats:p>","DOI":"10.1099\/jmm.0.001801","type":"journal-article","created":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T10:16:28Z","timestamp":1707128188000},"source":"Crossref","is-referenced-by-count":4,"title":["Efficacy evaluation of anti-DEC-IgY against antibiotic-resistant diarrhoeagenic Escherichia coli"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3243-3164","authenticated-orcid":false,"given":"Srichandrasekar Thuthikkadu","family":"Indhuprakash","sequence":"first","affiliation":[{"name":"Centre for Research in Infectious Diseases (CRID), Bioengineering, School of Chemical and Biotechnology, SASTRA Deemed-to-be-University, Thanjavur 613401, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panchapakesan","family":"S","sequence":"additional","affiliation":[{"name":"Central Animal Facility, 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