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Vaccination against Dengue virus is being introduced in some areas.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Discussion.<\/jats:bold> Appropriate diagnostic strategies will vary between laboratories depending on the available resources and skills. Diagnostic methods available include viral culture, the serological detection of Dengue-specific antibodies in using enzyme immunoassays (EIAs), microsphere immunoassays, haemagglutination inhibition or in lateral flow point of care tests. The results of antibody tests may be influenced by prior vaccination and exposure to other flaviviruses. The detection of non-structural protein 1 in serum (NS1) has improved the early diagnosis of Dengue and is available in point-of-care assays in addition to EIAs. Direct detection of viral RNA from blood by PCR is more sensitive than NS1 antigen detection but requires molecular skills and resources. An increasing variety of isothermal nucleic acid detection methods are in development. Timing of specimen collection and choice of test is critical to optimize diagnostic accuracy. Metagenomics and the direct detection by sequencing of viral RNA from blood offers the ability to rapidly type isolates for epidemiologic purposes.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> The impact of vaccination on immune response must be recognized as it will impact test interpretation and diagnostic algorithms.<\/jats:p>","DOI":"10.1099\/jmm.0.001833","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T15:17:32Z","timestamp":1715267852000},"source":"Crossref","is-referenced-by-count":26,"title":["Dengue: A review of laboratory diagnostics in the vaccine age"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7405-4499","authenticated-orcid":false,"given":"Jaimie L.","family":"Frazer","sequence":"first","affiliation":[{"name":"Pathology Queensland, Townsville QLD, 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