{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:09:53Z","timestamp":1747181393115,"version":"3.40.5"},"reference-count":51,"publisher":"Microbiology Society","issue":"7","funder":[{"DOI":"10.13039\/501100005417","name":"Universiti Teknologi Malaysia","doi-asserted-by":"crossref","award":["06E00"],"award-info":[{"award-number":["06E00"]}],"id":[{"id":"10.13039\/501100005417","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Gusu Innovation and Entrepreneurship Leading Talents Project","award":["ZXL2018192"],"award-info":[{"award-number":["ZXL2018192"]}]},{"name":"Jiangsu Province Industry-University-Research Cooperation Project","award":["BY2020506"],"award-info":[{"award-number":["BY2020506"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,5]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Cold plasma is frequently utilized for the purpose of eliminating microbial contaminants. Under optimal conditions, it can function as plasma medicine for treating various diseases, including infections caused by <jats:italic>Candida albicans<\/jats:italic>, an opportunistic pathogen that can overgrow in individuals with weakened immune system.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap Statement.<\/jats:bold> To date, there has been less molecular study on cold plasma-treated <jats:italic>C. albicans<\/jats:italic>.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Research Aim.<\/jats:bold> The study aims to fill the gap in understanding the molecular response of <jats:italic>C. albicans<\/jats:italic> to cold plasma treatment.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> This project involved testing a cold plasma generator to determine its antimicrobial effectiveness on <jats:italic>C. albicans<\/jats:italic>\u2019 planktonic cells. Additionally, the cells\u2019 transcriptomics responses were investigated using RNA sequencing at various treatment durations (1, 3 and 5\u2009min).<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> The results show that our cold plasma effectively eliminates <jats:italic>C. albicans<\/jats:italic>. Cold plasma treatment resulted in substantial downregulation of important pathways, such as \u2018nucleotide metabolism\u2019, \u2018DNA replication and repair\u2019, \u2018cell growth\u2019, \u2018carbohydrate metabolism\u2019 and \u2018amino acid metabolism\u2019. This was an indication of cell cycle arrest of <jats:italic>C. albicans<\/jats:italic> to preserve energy consumption under unfavourable conditions. Nevertheless, <jats:italic>C. albicans<\/jats:italic> adapted its GSH antioxidant system to cope with the oxidative stress induced by reactive oxygen species, reactive nitrogen species and other free radicals. The treatment likely led to a decrease in cell pathogenicity as many virulence factors were downregulated.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> The study demonstrated the major affected pathways in cold plasma-treated <jats:italic>C. albicans<\/jats:italic>, providing valuable insights into the molecular response of <jats:italic>C. albicans<\/jats:italic> to cold plasma treatment. The findings contribute to the understanding of the antimicrobial efficiency of cold plasma and its potential applications in the field of microbiology.<\/jats:p>","DOI":"10.1099\/jmm.0.001841","type":"journal-article","created":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T14:23:43Z","timestamp":1720189423000},"source":"Crossref","is-referenced-by-count":0,"title":["Transcriptome analysis of Candida albicans planktonic cells in response to plasma medicine"],"prefix":"10.1099","volume":"73","author":[{"given":"Xinhua","family":"Zhang","sequence":"first","affiliation":[{"name":"Jiangsu Huayu Printing & Coating Equipment Co. 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